{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hu L"],"funding":["National Natural Science Foundation of China"],"pagination":["111202"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12925561"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["302(3)"],"pubmed_abstract":["Endothelin-1 (ET-1) from renal-tubule-epithelial-cells inhibits NaCl reabsorption via ET<sub>B</sub> receptor in an autocrine manner, and inhibition of ET<sub>B</sub> receptors leads to salt-sensitive-hypertension. In the distal convoluted tubule (DCT), NaCl enters the cell via NaCl-cotransporter (NCC) and Cl<sup>-</sup> exits the cell in part by ClC-K2 channels, which play a role in regulating With-No-lysine kinase 4 (WNK4). The study aims to explore whether ET-1-induced inhibition of NaCl absorption is also achieved by inhibiting the basolateral Cl<sup>-</sup> channels in the DCT. Patch-clamp and immunoblotting assessed ET-1 effects on DCT Cl<sup>-</sup> channels and NCC. Immunofluorescence images detected ETB-receptor expression in parvalbumin-positive DCT. Application of ET-1 decreased"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Nitric oxide mediates ET-1-induced-inhibition of NPPB-sensitive Cl&lt;sup&gt;-&lt;/sup&gt; currents in the early distal convoluted tubule of the mouse kidney."],"pmcid":["PMC12925561"],"funding_grant_id":["81900652","32360215"],"pubmed_authors":["Wang M","You Y","Qiu H","Zhang H","Hu L","Meng X","Xiao A"],"additional_accession":[]},"is_claimable":false,"name":"Nitric oxide mediates ET-1-induced-inhibition of NPPB-sensitive Cl&lt;sup&gt;-&lt;/sup&gt; currents in the early distal convoluted tubule of the mouse kidney.","description":"Endothelin-1 (ET-1) from renal-tubule-epithelial-cells inhibits NaCl reabsorption via ET<sub>B</sub> receptor in an autocrine manner, and inhibition of ET<sub>B</sub> receptors leads to salt-sensitive-hypertension. In the distal convoluted tubule (DCT), NaCl enters the cell via NaCl-cotransporter (NCC) and Cl<sup>-</sup> exits the cell in part by ClC-K2 channels, which play a role in regulating With-No-lysine kinase 4 (WNK4). The study aims to explore whether ET-1-induced inhibition of NaCl absorption is also achieved by inhibiting the basolateral Cl<sup>-</sup> channels in the DCT. Patch-clamp and immunoblotting assessed ET-1 effects on DCT Cl<sup>-</sup> channels and NCC. Immunofluorescence images detected ETB-receptor expression in parvalbumin-positive DCT. Application of ET-1 decreased","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-16T15:15:16.996Z","creation":"2026-07-10T03:10:07.801Z"},"accession":"S-EPMC12925561","cross_references":{"pubmed":["41581878"],"doi":["10.1016/j.jbc.2026.111202"]}}