{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sarkar A"],"funding":["NIGMS NIH HHS"],"pagination":["2569-76"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12925628"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(15)"],"pubmed_abstract":["In this work we investigate concentration-enhanced enzyme activity assays in nanofluidic biomolecule concentrator chips which can be used to detect and study very low abundance enzymes from cell lysates and other low volume, low concentration samples. A mathematical model is developed for a mode of operation of the assay (J. H. Lee, B. D. Cosgrove, D. A. Lauffenburger and J. Han, J. Am. Chem. Soc., 2009, 131, 10340-10341) in which enzyme and substrate are concentrated together into a plug on chip which results in a non-linear enhancement of the reaction rate. Two reaction phases, an initial quadratic enzyme-limited phase and a later, linear substrate-limited phase, are predicted and then verified with experiments. It is determined that, in most practical situations, the reaction eventually"],"journal":["Lab on a chip"],"pubmed_title":["Non-linear and linear enhancement of enzymatic reaction kinetics using a biomolecule concentrator."],"pmcid":["PMC12925628"],"funding_grant_id":["P50-GM68762","P50 GM068762"],"pubmed_authors":["Sarkar A","Han J"],"additional_accession":[]},"is_claimable":false,"name":"Non-linear and linear enhancement of enzymatic reaction kinetics using a biomolecule concentrator.","description":"In this work we investigate concentration-enhanced enzyme activity assays in nanofluidic biomolecule concentrator chips which can be used to detect and study very low abundance enzymes from cell lysates and other low volume, low concentration samples. A mathematical model is developed for a mode of operation of the assay (J. H. Lee, B. D. Cosgrove, D. A. Lauffenburger and J. Han, J. Am. Chem. Soc., 2009, 131, 10340-10341) in which enzyme and substrate are concentrated together into a plug on chip which results in a non-linear enhancement of the reaction rate. Two reaction phases, an initial quadratic enzyme-limited phase and a later, linear substrate-limited phase, are predicted and then verified with experiments. It is determined that, in most practical situations, the reaction eventually","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Aug","modification":"2026-07-16T18:48:24.112Z","creation":"2026-07-12T03:08:30.195Z"},"accession":"S-EPMC12925628","cross_references":{"pubmed":["21677981"],"doi":["10.1039/c0lc00588f"]}}