{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang S"],"funding":["Howard Hughes Medical Institute","NIDDK NIH HHS","National Natural Science Foundation of China","China Postdoctoral Science Foundation"],"pagination":["125-140.e12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12927586"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["59(1)"],"pubmed_abstract":["During organ development, tissue stem cells first expand via symmetric divisions and then switch to asymmetric divisions to minimize the time to obtain a mature tissue. In the Drosophila midgut, intestinal stem cells switch their divisions from symmetric to asymmetric at midpupal development to produce enteroendocrine cells. However, the signals that initiate this switch are unknown. Here, we identify the signal as ecdysteroids. In the presence of ecdysone, EcR and Usp promote the expression of E93 to suppress Br expression, resulting in asymmetric divisions. Surprisingly, the primary source of pupal ecdysone is not from the prothoracic gland but from dorsal internal oblique muscles (DIOMs), a group of transient skeletal muscles that are required for eclosion. Genetic analysis shows that D"],"journal":["Developmental cell"],"pubmed_title":["Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila."],"pmcid":["PMC12927586"],"funding_grant_id":["555108552","R01 DK082456","32271074","31771625","31970817","2017M622408","31900618"],"pubmed_authors":["Guo Z","Wu S","Yao R","Wei X","Zhang S","Ohlstein B"],"additional_accession":[]},"is_claimable":false,"name":"Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila.","description":"During organ development, tissue stem cells first expand via symmetric divisions and then switch to asymmetric divisions to minimize the time to obtain a mature tissue. In the Drosophila midgut, intestinal stem cells switch their divisions from symmetric to asymmetric at midpupal development to produce enteroendocrine cells. However, the signals that initiate this switch are unknown. Here, we identify the signal as ecdysteroids. In the presence of ecdysone, EcR and Usp promote the expression of E93 to suppress Br expression, resulting in asymmetric divisions. Surprisingly, the primary source of pupal ecdysone is not from the prothoracic gland but from dorsal internal oblique muscles (DIOMs), a group of transient skeletal muscles that are required for eclosion. Genetic analysis shows that D","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jan","modification":"2026-07-16T17:45:04.719Z","creation":"2026-07-09T11:09:40.775Z"},"accession":"S-EPMC12927586","cross_references":{"pubmed":["38096823"],"doi":["10.1016/j.devcel.2023.11.016"]}}