{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ou HD"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA)","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NIBIB NIH HHS","Howard Hughes Medical Institute","NIDA NIH HHS","W. M. Keck Foundation","U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse","NCI NIH HHS","NIGMS NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)"],"pagination":["934-966"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12928153"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(4)"],"pubmed_abstract":["Structure determines function. The discovery of the DNA double-helix structure revealed how genetic information is stored and copied. In the mammalian cell nucleus, up to two meters of DNA is compacted by histones to form nucleosome/DNA particle chains that form euchromatin and heterochromatin domains, chromosome territories and mitotic chromosomes upon cell division. A critical question is what are the structures, interactions and 3D organization of DNA as chromatin in the nucleus and how do they determine DNA replication timing, gene expression and ultimately cell fate. To visualize genomic DNA across these different length scales in the nucleus, we developed ChromEMT, a method that selectively enhances the electron density and contrast of DNA and interacting nucleosome particles, which "],"journal":["Nature protocols"],"pubmed_title":["ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ."],"pmcid":["PMC12928153"],"funding_grant_id":["U01DA047731","P30 CA014195","U01 DA047731","GM103412","U01 EB021247","P41 GM103412"],"pubmed_authors":["Ellisman MH","Phan S","O'Shea CC","Ou HD","Inagaki A","Deerinck TJ"],"additional_accession":[]},"is_claimable":false,"name":"ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situ.","description":"Structure determines function. The discovery of the DNA double-helix structure revealed how genetic information is stored and copied. In the mammalian cell nucleus, up to two meters of DNA is compacted by histones to form nucleosome/DNA particle chains that form euchromatin and heterochromatin domains, chromosome territories and mitotic chromosomes upon cell division. A critical question is what are the structures, interactions and 3D organization of DNA as chromatin in the nucleus and how do they determine DNA replication timing, gene expression and ultimately cell fate. To visualize genomic DNA across these different length scales in the nucleus, we developed ChromEMT, a method that selectively enhances the electron density and contrast of DNA and interacting nucleosome particles, which ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-16T17:48:05.803Z","creation":"2026-07-09T11:09:53.804Z"},"accession":"S-EPMC12928153","cross_references":{"pubmed":["39613943"],"doi":["10.1038/s41596-024-01071-2"]}}