{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ali H"],"pubmed_abstract":["<h4>Background</h4>Glioblastoma (GBM) exhibits profound resistance to CD8⁺ T cell-mediated killing, yet the tumor-intrinsic mechanisms driving this immune evasion remain poorly defined. Our earlier study revealed Checkpoint Kinase 2 (Chek2) as the driver of CD8+ T cell resistance. This study investigates the immunomodulatory program exerted by the CHK2-YBX1&YBX3 regulatory hub.<h4>Methods</h4>Protein-protein interactions were investigated through immunoprecipitation (IP) followed by mass spectrometry (MS) and phosphoproteomics. Single gene knockout of CHEK2, Y-box-binding protein 1 (YBX1), and Y-box-binding protein 3 (YBX3) were generated in human and mouse glioma cells. Transcriptomic and epigenetic alterations were characterized by bulk RNA sequencing and chromatin immunoprecipitation se"],"journal":["Neuro-oncology"],"pagination":["noaf227"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12930254"],"repository":["biostudies-literature"],"pubmed_title":["YBX1&amp;YBX3 as novel targets to potentiate immune checkpoint blockade response in gliomas."],"pmcid":["PMC12930254"],"pubmed_authors":["Ali H","Lee-Chang C","Zhou N","Tulaiha R","Dmello C","Yeeravalli R","Henrik Heiland D","Chen L","Mishra S","Habashy K","van Hijfte L","Sonabend AM","Zhou Y","Kim KS","Duffy J","Wang L","Tiek DM","Horbinski C","Ahmed A","Karri V","Song X","Arrieta VA"],"additional_accession":[]},"is_claimable":false,"name":"YBX1&amp;YBX3 as novel targets to potentiate immune checkpoint blockade response in gliomas.","description":"<h4>Background</h4>Glioblastoma (GBM) exhibits profound resistance to CD8⁺ T cell-mediated killing, yet the tumor-intrinsic mechanisms driving this immune evasion remain poorly defined. Our earlier study revealed Checkpoint Kinase 2 (Chek2) as the driver of CD8+ T cell resistance. This study investigates the immunomodulatory program exerted by the CHK2-YBX1&YBX3 regulatory hub.<h4>Methods</h4>Protein-protein interactions were investigated through immunoprecipitation (IP) followed by mass spectrometry (MS) and phosphoproteomics. Single gene knockout of CHEK2, Y-box-binding protein 1 (YBX1), and Y-box-binding protein 3 (YBX3) were generated in human and mouse glioma cells. Transcriptomic and epigenetic alterations were characterized by bulk RNA sequencing and chromatin immunoprecipitation se","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-07-09T12:23:30.552Z","creation":"2026-07-09T11:17:43.335Z"},"accession":"S-EPMC12930254","cross_references":{"pubmed":["41025501"],"doi":["10.1093/neuonc/noaf227"]}}