biostudies-literatureHuang GNational Key Wildlife Conservation Project7564https://www.ebi.ac.uk/biostudies/studies/S-EPMC12932853biostudies-literatureUnknown16(1)Soil microorganisms are essential for sustaining ecosystem functions, driving biogeochemical cycles, and modulating carbon storage. However, the nutrient-mediated mechanisms by which different land-use types shape soil microbial communities remain unclear. This study investigated three typical land-use types-plantation, grassland, and high-standard cropland-in Xinyang City, China, to evaluate their effects on soil microbial community. Results showed that soil nutrient contents-including total nitrogen, total phosphorus, alkaline-hydrolyzable nitrogen, and available phosphorus-as well as microbial alpha diversity indices, were consistently higher in topsoil than in subsoil and more pronounced in plantation than in grassland and cropland. Acidobacteriota, Pseudomonadota, Ascomycota, and BasiScientific reportsInfluence of land-use types on soil microbial communities and nutrient changes in Xinyang City, China.PMC12932853GTH [2024]153Rong YHuang SGuan ZHuang GHuang XSong CMa TfalseInfluence of land-use types on soil microbial communities and nutrient changes in Xinyang City, China.Soil microorganisms are essential for sustaining ecosystem functions, driving biogeochemical cycles, and modulating carbon storage. However, the nutrient-mediated mechanisms by which different land-use types shape soil microbial communities remain unclear. This study investigated three typical land-use types-plantation, grassland, and high-standard cropland-in Xinyang City, China, to evaluate their effects on soil microbial community. Results showed that soil nutrient contents-including total nitrogen, total phosphorus, alkaline-hydrolyzable nitrogen, and available phosphorus-as well as microbial alpha diversity indices, were consistently higher in topsoil than in subsoil and more pronounced in plantation than in grassland and cropland. Acidobacteriota, Pseudomonadota, Ascomycota, and Basi2026-01-01T00:00:00Z2026 Feb2026-07-16T20:47:32.345Z2026-07-10T03:12:41.395ZS-EPMC129328534165192610.1038/s41598-026-38635-z