{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pott ED"],"funding":["University of Arkansas","Society for Analytical Chemists of Pittsburgh","Arkansas Biosciences Institute"],"pagination":["788-794"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12933340"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(2)"],"pubmed_abstract":["Simultaneous determination of spatial location and spectral color of single molecules at large molecular density with high throughput was achieved by combining single-molecule photoswitching and optical in-hardware Fourier transformation of single-molecule emission spectra into the phasor space. The method, named as spectral phasor enabled stochastic optical reconstruction microscopy (SP-STORM), achieved simultaneous super-resolution imaging of five subcellular structures in parallel with minimum crosstalk for the first time. The high-throughput feature of SP-STORM enables these subcellular structures to be readily resolved in about one min, which is more than an order of magnitude faster than other multiplexing single-molecule localization microscopy techniques. The concept of SP-STORM is"],"journal":["JACS Au"],"pubmed_title":["High-Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM."],"pmcid":["PMC12933340"],"funding_grant_id":["PG105140"],"pubmed_authors":["Batey JE","Pott ED","Yang M","Embree J","Dong B"],"additional_accession":[]},"is_claimable":false,"name":"High-Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM.","description":"Simultaneous determination of spatial location and spectral color of single molecules at large molecular density with high throughput was achieved by combining single-molecule photoswitching and optical in-hardware Fourier transformation of single-molecule emission spectra into the phasor space. The method, named as spectral phasor enabled stochastic optical reconstruction microscopy (SP-STORM), achieved simultaneous super-resolution imaging of five subcellular structures in parallel with minimum crosstalk for the first time. The high-throughput feature of SP-STORM enables these subcellular structures to be readily resolved in about one min, which is more than an order of magnitude faster than other multiplexing single-molecule localization microscopy techniques. The concept of SP-STORM is","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T22:11:23.075Z","creation":"2026-07-14T03:03:42.233Z"},"accession":"S-EPMC12933340","cross_references":{"pubmed":["41755849"],"doi":["10.1021/jacsau.5c01677"]}}