biostudies-literatureChen YShanghai Songjiang District Research FoundationBasic and Applied Basic Research Foundation of Guangdong ProvinceNational Natural Science Foundation of China21https://www.ebi.ac.uk/biostudies/studies/S-EPMC12933926biostudies-literatureUnknown33(1)<h4>Background</h4>Autoimmune inflammation results from dysregulated immune responses, with dysfunction of regulatory T cells (Tregs) being a key contributor due to their critical role in maintaining immune tolerance. The stability and function of Tregs are strongly influenced by the inflammatory microenvironment, yet the regulatory interactions between CD8⁺ T cells and CD4⁺Foxp3⁺ Tregs remain poorly understood.<h4>Methods</h4>We investigated the role of CD8⁺ T cells in regulating induced Tregs (iTregs) using in vitro T cell co-culture assays and two in vivo models of autoimmune disease. A naïve CD4⁺ T cell transfer colitis model was used to evaluate the suppressive function of iTregs in the presence or absence of CD8⁺ T celJournal of biomedical scienceTGF-β signaling mediates crosstalk between CD8&lt;sup&gt;+&lt;/sup&gt; T cells and CD39&lt;sup&gt;+&lt;/sup&gt; induced Treg cells in autoimmune inflammation.PMC1293392682371817820017272024SJKJGG0592022A1515111173818712242024SJKJGG076Liang RZChen JRLiu LLu YDang JLZhao WXiong YDZheng SGLong QWang JZang NYang YOlsen NChen YZhao JPan YFfalseTGF-β signaling mediates crosstalk between CD8&lt;sup&gt;+&lt;/sup&gt; T cells and CD39&lt;sup&gt;+&lt;/sup&gt; induced Treg cells in autoimmune inflammation.<h4>Background</h4>Autoimmune inflammation results from dysregulated immune responses, with dysfunction of regulatory T cells (Tregs) being a key contributor due to their critical role in maintaining immune tolerance. The stability and function of Tregs are strongly influenced by the inflammatory microenvironment, yet the regulatory interactions between CD8⁺ T cells and CD4⁺Foxp3⁺ Tregs remain poorly understood.<h4>Methods</h4>We investigated the role of CD8⁺ T cells in regulating induced Tregs (iTregs) using in vitro T cell co-culture assays and two in vivo models of autoimmune disease. A naïve CD4⁺ T cell transfer colitis model was used to evaluate the suppressive function of iTregs in the presence or absence of CD8⁺ T cel2026-01-01T00:00:00Z2026 Feb2026-07-16T20:49:36.639Z2026-07-10T03:12:05.327ZS-EPMC129339264173607110.1186/s12929-026-01228-z