biostudies-literatureUnknownSharma CNational Heart, Lung, and Blood InstituteNational Institutes of HealthWe present a novel one-step methodology for direct mass spectrometric characterization of sulfated non-saccharide glycosaminoglycan mimetics in their sodiated form using the high reactivity of trifluorodiazoethane to form hydrophobic and chemically stable esters that facilitates separation and detection in a liquid chromatography-mass spectrometry system. The methodology preserves all sulfates present in the parent molecule, thereby allowing for accurate mass characterization of the per-sulfated molecule.Analytical methods : advancing methods and applicationshttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12934201biostudies-literatureA one-step chemical derivatization strategy for mass spectrometric characterization of synthetic mimetics of sulfated glycosaminoglycans.PMC12934201P01 HL151333R00 HL161423R61/33 HL161813Sharma CAfosah DKDesai URVilluri BKfalseA one-step chemical derivatization strategy for mass spectrometric characterization of synthetic mimetics of sulfated glycosaminoglycans.We present a novel one-step methodology for direct mass spectrometric characterization of sulfated non-saccharide glycosaminoglycan mimetics in their sodiated form using the high reactivity of trifluorodiazoethane to form hydrophobic and chemically stable esters that facilitates separation and detection in a liquid chromatography-mass spectrometry system. The methodology preserves all sulfates present in the parent molecule, thereby allowing for accurate mass characterization of the per-sulfated molecule.2026-01-01T00:00:00Z2026 Feb2026-07-10T03:24:20.671Z2026-07-10T03:16:12.383ZS-EPMC129342014173862510.1039/d5ay01855b