biostudies-literatureUnknownEllin NRNational Institute of General Medical SciencesThis work summarizes the criteria for a useful reagent for ion/ion proton transfer in an electrodynamic ion trap with specific emphasis on proton transfer to high mass multiply-charged analyte ions. A readily available and ionizable weak base of relatively high mass meets the criteria. The protonated form of an extensively fluorinated 10-carbon primary amine (1<i>H</i>,1<i>H</i>,2<i>H</i>,2<i>H</i>-perfluorodecylamine, PFDA) is demonstrated to serve as a useful reagent for proton transfer to negatively-charged proteins and oligonucleotides. The behavior of protonated PFDA is compared with that of protonated 1,8-bis(dimethylamino)naphthalene (proton sponge) with respect to the tendency for proton transfer <i>versus</i> attachment in ion/ion reactions with a common multiply-deprotonated protThe Analysthttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12935111biostudies-literatureProton transfer reagent cations for ion-ion charge state manipulation of high mass negatively-charged analytes in an electrodynamic ion trap.PMC12935111R35-153191McLuckey SAFaye BKSHorn SAKoers AMEllin NRfalseProton transfer reagent cations for ion-ion charge state manipulation of high mass negatively-charged analytes in an electrodynamic ion trap.This work summarizes the criteria for a useful reagent for ion/ion proton transfer in an electrodynamic ion trap with specific emphasis on proton transfer to high mass multiply-charged analyte ions. A readily available and ionizable weak base of relatively high mass meets the criteria. The protonated form of an extensively fluorinated 10-carbon primary amine (1<i>H</i>,1<i>H</i>,2<i>H</i>,2<i>H</i>-perfluorodecylamine, PFDA) is demonstrated to serve as a useful reagent for proton transfer to negatively-charged proteins and oligonucleotides. The behavior of protonated PFDA is compared with that of protonated 1,8-bis(dimethylamino)naphthalene (proton sponge) with respect to the tendency for proton transfer <i>versus</i> attachment in ion/ion reactions with a common multiply-deprotonated prot2026-01-01T00:00:00Z2026 Feb2026-07-16T20:47:54.576Z2026-07-10T03:13:06.457ZS-EPMC129351114173964810.1039/d5an01354b