biostudies-literatureCarvalho ARVConselho Nacional de Desenvolvimento Científico e Tecnológicoe03147https://www.ebi.ac.uk/biostudies/studies/S-EPMC12935167biostudies-literatureUnknown23(2)Widely used in Brazilian folk medicine, Costus arabicus L. (Canarana) is a herbaceous plant with promising pharmacological potential. Here, we profile the molecular composition of extracts from different plant organs and evaluate their in vitro activity against Zika virus (ZIKV). Ethanolic and aqueous extracts from leaves, stems, and roots were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for metabolite assignment. In vitro antiviral activity was assessed against Zika, Chikungunya, and Oropouche viruses. Forty-five compounds were annotated, including amino acids, flavones, flavonols, glycosylated flavonoids, lignans, lipids, phenolic acids, steroidal saponins, and xanthones. The ethanolic extract demonstrated promising anti-ZIKV activity, with an EC₅₀ ofChemistry & biodiversityUnveiling the Molecular Profile and Antiviral Potential of Costus arabicus L. Against Zika Virus.PMC12935167155028/2022-7Ferraz ACda Silva Menegatto MBda C Lopes JCarvalho ARVPamplona SDGSRYoshioka E Silva CYde Brito Magalhaes CLda Silva MNReis JDEGomes PWPfalseUnveiling the Molecular Profile and Antiviral Potential of Costus arabicus L. Against Zika Virus.Widely used in Brazilian folk medicine, Costus arabicus L. (Canarana) is a herbaceous plant with promising pharmacological potential. Here, we profile the molecular composition of extracts from different plant organs and evaluate their in vitro activity against Zika virus (ZIKV). Ethanolic and aqueous extracts from leaves, stems, and roots were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for metabolite assignment. In vitro antiviral activity was assessed against Zika, Chikungunya, and Oropouche viruses. Forty-five compounds were annotated, including amino acids, flavones, flavonols, glycosylated flavonoids, lignans, lipids, phenolic acids, steroidal saponins, and xanthones. The ethanolic extract demonstrated promising anti-ZIKV activity, with an EC₅₀ of2026-01-01T00:00:00Z2026 Feb2026-07-16T21:34:51.874Z2026-07-12T03:09:13.661ZS-EPMC129351674173971610.1002/cbdv.202503147