biostudies-literatureMonteiro JFAPESPe03280https://www.ebi.ac.uk/biostudies/studies/S-EPMC12935287biostudies-literatureUnknown23(2)In this study, the chemical composition of essential oils (EOs) extracted from leaves of Duguetia lanceolata collected in different seasons (autumn and winter) was analyzed using a GNPS untargeted GC-MS metabolomic approach. EO from autumn leaves (EO-A) contained 80 metabolites (93.8%), with limonene (28.5%), (+)-β-pinene (15.0%), cyclocolorenone (12.8%), 6,9-guaiadiene (3.1%), and 4-carvomenthenol (3.1%) as major constituents. EO from winter leaves (EO-W) comprised 102 compounds (88.0%), dominated by β-bisabolene (16.4%), β-gurjunene (6.4%), β-caryophyllene (4.7%), cyclocolorenone (3.9%), and dihydrocarveol (3.3%). The antimicrobial activity of both EOs was evaluated in vitro using the disk diffusion method against gram-positive and gram-negative bacteria, as well as selected yeast strainChemistry & biodiversityGNPS Untargeted GC-MS Metabolomic Analysis of Essential Oils From Duguetia lanceolata and Evaluation of Antimicrobial Activity.PMC12935287#2024/18638-6#2022/09202-4#2021/10599-3#2015/04400-9#2020/01000-8Grecco SSVallim MALago JHGSoares MGAntonelli GNSartorelli PDantas EPMonteiro JPascon RCfalseGNPS Untargeted GC-MS Metabolomic Analysis of Essential Oils From Duguetia lanceolata and Evaluation of Antimicrobial Activity.In this study, the chemical composition of essential oils (EOs) extracted from leaves of Duguetia lanceolata collected in different seasons (autumn and winter) was analyzed using a GNPS untargeted GC-MS metabolomic approach. EO from autumn leaves (EO-A) contained 80 metabolites (93.8%), with limonene (28.5%), (+)-β-pinene (15.0%), cyclocolorenone (12.8%), 6,9-guaiadiene (3.1%), and 4-carvomenthenol (3.1%) as major constituents. EO from winter leaves (EO-W) comprised 102 compounds (88.0%), dominated by β-bisabolene (16.4%), β-gurjunene (6.4%), β-caryophyllene (4.7%), cyclocolorenone (3.9%), and dihydrocarveol (3.3%). The antimicrobial activity of both EOs was evaluated in vitro using the disk diffusion method against gram-positive and gram-negative bacteria, as well as selected yeast strain2026-01-01T00:00:00Z2026 Feb2026-07-16T21:32:33.468Z2026-07-10T03:16:24.468ZS-EPMC129352874173980510.1002/cbdv.202503280