biostudies-literatureAgid NNatural Sciences and Engineering Research Council of CanadaNational Heart, Lung, and Blood InstituteNational Institutes of HealthBlair FoundationCanadian Institutes of Health Research6https://www.ebi.ac.uk/biostudies/studies/S-EPMC12935489biostudies-literatureUnknown42<h4>Purpose</h4>This study aimed to investigate the relationship between the two biomarkers, von Willebrand factor (VWF) and ADAMTS13, and major adverse limb events (MALE) in patients with peripheral artery disease (PAD).<h4>Materials and methods</h4>After obtaining informed consent, baseline blood samples were collected from 48 PAD patients aged 60-75 years who were undergoing assessment, surveillance, medical management, or consideration for surgical intervention. VWF and ADAMTS13 antigen levels were measured by enzyme-linked immunosorbent assay (ELISA). Patients were monitored prospectively for subsequent MALE, defined as lower-limb revascularization or major amputation. Biomarker levels were compared between patients who did and did not experience subsequent MALE using Mann-Whitney U-tVascular specialist internationalVon Willebrand Factor and ADAMTS13 in Relation to Major Adverse Limb Events in Peripheral Artery Disease.PMC12935489R01HL172780Andrisani PKhan HBhagirath VCAgid NKretz CAQadura MfalseVon Willebrand Factor and ADAMTS13 in Relation to Major Adverse Limb Events in Peripheral Artery Disease.<h4>Purpose</h4>This study aimed to investigate the relationship between the two biomarkers, von Willebrand factor (VWF) and ADAMTS13, and major adverse limb events (MALE) in patients with peripheral artery disease (PAD).<h4>Materials and methods</h4>After obtaining informed consent, baseline blood samples were collected from 48 PAD patients aged 60-75 years who were undergoing assessment, surveillance, medical management, or consideration for surgical intervention. VWF and ADAMTS13 antigen levels were measured by enzyme-linked immunosorbent assay (ELISA). Patients were monitored prospectively for subsequent MALE, defined as lower-limb revascularization or major amputation. Biomarker levels were compared between patients who did and did not experience subsequent MALE using Mann-Whitney U-t2026-01-01T00:00:00Z2026 Feb2026-07-16T21:36:14.15Z2026-07-10T03:15:11.757ZS-EPMC129354894172537210.5758/vsi.250103