{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Uruci S"],"funding":["Swiss National Science Foundation","European Research Council"],"pagination":["1025-1034"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12935552"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["650(8103)"],"pubmed_abstract":["Fine-tuning DNA replication and transcription is crucial to prevent collisions between their machineries<sup>1</sup>. This is particularly important near promoters, where RNA polymerase II (RNAPII) initiates transcription and frequently arrests, forming R-loops<sup>2-4</sup>. Arrested RNAPII can obstruct DNA replication, which often initiates near promoters<sup>5,6</sup>. The mechanisms that rescue arrested RNAPII during elongation to avoid conflicts with co-directional replisomes remain unclear. Here, using genome-wide approaches and genetic screens, we identify CFAP20 as part of a protective pathway that salvages arrested RNAPII in promoter-proximal regions, diverting it from the path of co-directional replisomes. CFAP20-deficient cells accumulate R-loops near promoters, which leads to d"],"journal":["Nature"],"pubmed_title":["CFAP20 salvages arrested RNAPII from the path of co-directional replisomes."],"pmcid":["PMC12935552"],"funding_grant_id":["852798","310913","101053581","197003","101043815"],"pubmed_authors":["Wendel TJ","Tropepe V","de Lint K","Panagopoulos A","Wolthuis RMF","Boer DEC","van Attikum H","Wondergem AP","Vertegaal ACO","Kirdok I","Altmeyer M","van Oudenaarden A","Lingeman J","Ljungman M","van den Heuvel D","Uruci S","Brussee SJ","Hamperl S","Lalonde M","Yakoub G","Chrystal PW","Noordermeer SM","Luijsterburg MS","van den Berg J","van der Woude M","van Overbeek NK","Schotman N"],"additional_accession":[]},"is_claimable":false,"name":"CFAP20 salvages arrested RNAPII from the path of co-directional replisomes.","description":"Fine-tuning DNA replication and transcription is crucial to prevent collisions between their machineries<sup>1</sup>. This is particularly important near promoters, where RNA polymerase II (RNAPII) initiates transcription and frequently arrests, forming R-loops<sup>2-4</sup>. Arrested RNAPII can obstruct DNA replication, which often initiates near promoters<sup>5,6</sup>. The mechanisms that rescue arrested RNAPII during elongation to avoid conflicts with co-directional replisomes remain unclear. Here, using genome-wide approaches and genetic screens, we identify CFAP20 as part of a protective pathway that salvages arrested RNAPII in promoter-proximal regions, diverting it from the path of co-directional replisomes. CFAP20-deficient cells accumulate R-loops near promoters, which leads to d","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T21:56:54.676Z","creation":"2026-07-10T03:15:56.349Z"},"accession":"S-EPMC12935552","cross_references":{"pubmed":["41535461"],"doi":["10.1038/s41586-025-09943-7"]}}