{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["110(1)"],"submitter":["Gao W"],"pubmed_abstract":["Methanol has emerged as a promising substrate in biomanufacturing, where high tolerance and efficient assimilation are critical to overcome the cytotoxic effects of methanol and its intermediate metabolites. In this study, a Pichia kudriavzevii P22 strain (genome size: 17.3 Mb) was isolated from fermented camel milk. Remarkably, this strain tolerated up to 15% (v/v) methanol and survived under multiple stress conditions, including pH 2, 47 °C, 5 g/L acetic acid, and 100 g/L NaCl. In a 5-L fed-batch fermentation system operated under open, non-sterile conditions with 7.5% (v/v) methanol as the sole carbon source, P22 remained viable for 78 h. The maximum cell dry weight (CDW) reached 27.56 g/L, with proteins accounting for 54%. Furthermore, a preliminary investigation of the methanol metabo"],"journal":["Applied microbiology and biotechnology"],"pagination":["77"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12935861"],"repository":["biostudies-literature"],"pubmed_title":["Robust Pichia kudriavzevii for open production of methylotrophic protein."],"pmcid":["PMC12935861"],"pubmed_authors":["Yin G","Wang R","Gao W","Zhang M","Chen H","Wang Y","Yue H","Xu J","Kuang Z","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Robust Pichia kudriavzevii for open production of methylotrophic protein.","description":"Methanol has emerged as a promising substrate in biomanufacturing, where high tolerance and efficient assimilation are critical to overcome the cytotoxic effects of methanol and its intermediate metabolites. In this study, a Pichia kudriavzevii P22 strain (genome size: 17.3 Mb) was isolated from fermented camel milk. Remarkably, this strain tolerated up to 15% (v/v) methanol and survived under multiple stress conditions, including pH 2, 47 °C, 5 g/L acetic acid, and 100 g/L NaCl. In a 5-L fed-batch fermentation system operated under open, non-sterile conditions with 7.5% (v/v) methanol as the sole carbon source, P22 remained viable for 78 h. The maximum cell dry weight (CDW) reached 27.56 g/L, with proteins accounting for 54%. Furthermore, a preliminary investigation of the methanol metabo","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-16T22:04:34.812Z","creation":"2026-07-11T03:11:24.708Z"},"accession":"S-EPMC12935861","cross_references":{"pubmed":["41735566"],"doi":["10.1007/s00253-026-13745-w"]}}