{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Silva-Espinoza JC"],"funding":["NIH HHS"],"pagination":["16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12938254"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["<b>Background/Objectives</b>: Schlafen (SLFN) 8 and SLFN9 are mouse members of the Schlafen protein family, believed to have arisen through a gene duplication event. The physiological roles of these proteins remain poorly defined, in part due to the absence of reliable, commercially available antibodies for their detection. <b>Methods</b>: To develop specific antibodies, we performed an amino acid sequence alignment of these proteins and identified a thirteen amino acids long peptide predicted by AlphaFold modeling and hydropathicity analysis to be surface-exposed in both SLFN proteins. The SLFN8 peptide was conjugated to KLH and used to immunize mice, employing Poly(I:C) as an adjuvant. <b>Results</b>: We verified the anti-SLFN8 antibody specificity in mouse tissues, engineered human cell"],"journal":["Antibodies (Basel, Switzerland)"],"pubmed_title":["Generation of Schlafen 8-Specific Antibodies."],"pmcid":["PMC12938254"],"funding_grant_id":["R16AI167830","5SC1GM136630"],"pubmed_authors":["Llano M","Silva-Espinoza JC","Terrazas BA","Palos Vargas S","Carlin A","Rodriguez Rodriguez MI","Valenzuela C","Francia G","Prospero DL","Perucho CE"],"additional_accession":[]},"is_claimable":false,"name":"Generation of Schlafen 8-Specific Antibodies.","description":"<b>Background/Objectives</b>: Schlafen (SLFN) 8 and SLFN9 are mouse members of the Schlafen protein family, believed to have arisen through a gene duplication event. The physiological roles of these proteins remain poorly defined, in part due to the absence of reliable, commercially available antibodies for their detection. <b>Methods</b>: To develop specific antibodies, we performed an amino acid sequence alignment of these proteins and identified a thirteen amino acids long peptide predicted by AlphaFold modeling and hydropathicity analysis to be surface-exposed in both SLFN proteins. The SLFN8 peptide was conjugated to KLH and used to immunize mice, employing Poly(I:C) as an adjuvant. <b>Results</b>: We verified the anti-SLFN8 antibody specificity in mouse tissues, engineered human cell","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-10T03:19:11.413Z","creation":"2026-07-10T03:15:16.485Z"},"accession":"S-EPMC12938254","cross_references":{"pubmed":["41744902"],"doi":["10.3390/antib15010016"]}}