biostudies-literatureSilva-Espinoza JCNIH HHS16https://www.ebi.ac.uk/biostudies/studies/S-EPMC12938254biostudies-literatureUnknown15(1)<b>Background/Objectives</b>: Schlafen (SLFN) 8 and SLFN9 are mouse members of the Schlafen protein family, believed to have arisen through a gene duplication event. The physiological roles of these proteins remain poorly defined, in part due to the absence of reliable, commercially available antibodies for their detection. <b>Methods</b>: To develop specific antibodies, we performed an amino acid sequence alignment of these proteins and identified a thirteen amino acids long peptide predicted by AlphaFold modeling and hydropathicity analysis to be surface-exposed in both SLFN proteins. The SLFN8 peptide was conjugated to KLH and used to immunize mice, employing Poly(I:C) as an adjuvant. <b>Results</b>: We verified the anti-SLFN8 antibody specificity in mouse tissues, engineered human cellAntibodies (Basel, Switzerland)Generation of Schlafen 8-Specific Antibodies.PMC12938254R16AI1678305SC1GM136630Llano MSilva-Espinoza JCTerrazas BAPalos Vargas SCarlin ARodriguez Rodriguez MIValenzuela CFrancia GProspero DLPerucho CEfalseGeneration of Schlafen 8-Specific Antibodies.<b>Background/Objectives</b>: Schlafen (SLFN) 8 and SLFN9 are mouse members of the Schlafen protein family, believed to have arisen through a gene duplication event. The physiological roles of these proteins remain poorly defined, in part due to the absence of reliable, commercially available antibodies for their detection. <b>Methods</b>: To develop specific antibodies, we performed an amino acid sequence alignment of these proteins and identified a thirteen amino acids long peptide predicted by AlphaFold modeling and hydropathicity analysis to be surface-exposed in both SLFN proteins. The SLFN8 peptide was conjugated to KLH and used to immunize mice, employing Poly(I:C) as an adjuvant. <b>Results</b>: We verified the anti-SLFN8 antibody specificity in mouse tissues, engineered human cell2026-01-01T00:00:00Z2026 Feb2026-07-10T03:19:11.413Z2026-07-10T03:15:16.485ZS-EPMC129382544174490210.3390/antib15010016