biostudies-literatureZhang Cthe Science and Technology Commission of Shanghai Municipality ProjectEquipment research and development projects of the Chinese Academy of SciencesXiangfu Lab Research ProjectQinghai Provincial Department of Science and Technology Key Research and Development and Transformation ProgramNational Key Research and Development Program of China89https://www.ebi.ac.uk/biostudies/studies/S-EPMC12938609biostudies-literatureUnknown16(2)Tetrodotoxin (TTX) is a potent marine neurotoxin, necessitating sensitive and user-friendly on-site assays. To address long workflows of traditional immunoassays and limited signal amplification in colorimetric microfluidics, we developed a nanozyme-catalyzed colorimetric magnetic microfluidic immunosensor (Nano-CMI). This platform combines an aptamer-antibody sandwich capture format with catalytic amplification via AuNR@Pt@m-SiO₂ (APMS) nanozymes on a magnetically actuated microfluidic chip. Magnetic actuation simplifies sample handling and washing, while APMS catalysis enhances sensitivity and visual readout. The Nano-CMI has been used for the detection of TTX samples ranging from 0.2 to 20 ng/mL with a detection limit of 0.2 ng/mL in 10 min, following the linear equation: y =BiosensorsNanozyme-Based Colorimetric Assay on a Magnetic Microfluidic Platform for Integrated Detection of TTX.PMC12938609XTCX-KJ-2024-0382025-QY-2082023YFA0915200PTYQ2024BJ0007XF052025B0300PTYQ2024YZ00102023YFA0915204XF012023D0200Zhang FWu SLiu BZhang CChen CZhao JFeng SfalseNanozyme-Based Colorimetric Assay on a Magnetic Microfluidic Platform for Integrated Detection of TTX.Tetrodotoxin (TTX) is a potent marine neurotoxin, necessitating sensitive and user-friendly on-site assays. To address long workflows of traditional immunoassays and limited signal amplification in colorimetric microfluidics, we developed a nanozyme-catalyzed colorimetric magnetic microfluidic immunosensor (Nano-CMI). This platform combines an aptamer-antibody sandwich capture format with catalytic amplification via AuNR@Pt@m-SiO₂ (APMS) nanozymes on a magnetically actuated microfluidic chip. Magnetic actuation simplifies sample handling and washing, while APMS catalysis enhances sensitivity and visual readout. The Nano-CMI has been used for the detection of TTX samples ranging from 0.2 to 20 ng/mL with a detection limit of 0.2 ng/mL in 10 min, following the linear equation: y =2026-01-01T00:00:00Z2026 Feb2026-07-11T03:16:35.512Z2026-07-11T03:09:17.932ZS-EPMC129386094174470910.3390/bios16020089