{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Currie J"],"funding":["NIA NIH HHS","National Institutes of Health","Alzheimer&apos;s Association"],"pagination":["110219"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12940509"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["409"],"pubmed_abstract":["<h4>Background</h4>Study of synaptic integrity using conventional electrophysiology is a gold standard for quantitative assessment of neurodegeneration. Fluorescence assisted single-synapse long-term potentiation (FASS-LTP) provides a high throughput method to assess the synaptic integrity of neurotransmission within and between different brain regions as a measure of pharmacological efficacy in translational models.<h4>New method</h4>We adapted the existing method to our purpose by adding a step during the thawing of frozen samples, by an extra step of placing them on a rocker at room temperature for 30 minutes immediately following thawing with constant mixing on a shaker. This allowed for gradual, uniform thawing, effectively separating the synaptosomes. Our study demonstrates FASS-LTP "],"journal":["Journal of neuroscience methods"],"pubmed_title":["Using FASS-LTP in postmortem mice brain tissues to assess pathological synaptic function."],"pmcid":["PMC12940509"],"funding_grant_id":["R01 AG063945","R21 AG059223","T32 AG067952"],"pubmed_authors":["Budhwani S","Garza KH","Mallipudi M","Sreenivasamurthy SGS","Natarajan C","Currie J","Krishnan B"],"additional_accession":[]},"is_claimable":false,"name":"Using FASS-LTP in postmortem mice brain tissues to assess pathological synaptic function.","description":"<h4>Background</h4>Study of synaptic integrity using conventional electrophysiology is a gold standard for quantitative assessment of neurodegeneration. Fluorescence assisted single-synapse long-term potentiation (FASS-LTP) provides a high throughput method to assess the synaptic integrity of neurotransmission within and between different brain regions as a measure of pharmacological efficacy in translational models.<h4>New method</h4>We adapted the existing method to our purpose by adding a step during the thawing of frozen samples, by an extra step of placing them on a rocker at room temperature for 30 minutes immediately following thawing with constant mixing on a shaker. This allowed for gradual, uniform thawing, effectively separating the synaptosomes. Our study demonstrates FASS-LTP ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Sep","modification":"2026-07-16T21:57:23.047Z","creation":"2026-07-11T03:08:31.142Z"},"accession":"S-EPMC12940509","cross_references":{"pubmed":["39013526"],"doi":["10.1016/j.jneumeth.2024.110219"]}}