biostudies-literatureCurrie JNIA NIH HHSNational Institutes of HealthAlzheimer&apos;s Association110219https://www.ebi.ac.uk/biostudies/studies/S-EPMC12940509biostudies-literatureUnknown409<h4>Background</h4>Study of synaptic integrity using conventional electrophysiology is a gold standard for quantitative assessment of neurodegeneration. Fluorescence assisted single-synapse long-term potentiation (FASS-LTP) provides a high throughput method to assess the synaptic integrity of neurotransmission within and between different brain regions as a measure of pharmacological efficacy in translational models.<h4>New method</h4>We adapted the existing method to our purpose by adding a step during the thawing of frozen samples, by an extra step of placing them on a rocker at room temperature for 30 minutes immediately following thawing with constant mixing on a shaker. This allowed for gradual, uniform thawing, effectively separating the synaptosomes. Our study demonstrates FASS-LTP Journal of neuroscience methodsUsing FASS-LTP in postmortem mice brain tissues to assess pathological synaptic function.PMC12940509R01 AG063945R21 AG059223T32 AG067952Budhwani SGarza KHMallipudi MSreenivasamurthy SGSNatarajan CCurrie JKrishnan BfalseUsing FASS-LTP in postmortem mice brain tissues to assess pathological synaptic function.<h4>Background</h4>Study of synaptic integrity using conventional electrophysiology is a gold standard for quantitative assessment of neurodegeneration. Fluorescence assisted single-synapse long-term potentiation (FASS-LTP) provides a high throughput method to assess the synaptic integrity of neurotransmission within and between different brain regions as a measure of pharmacological efficacy in translational models.<h4>New method</h4>We adapted the existing method to our purpose by adding a step during the thawing of frozen samples, by an extra step of placing them on a rocker at room temperature for 30 minutes immediately following thawing with constant mixing on a shaker. This allowed for gradual, uniform thawing, effectively separating the synaptosomes. Our study demonstrates FASS-LTP 2024-01-01T00:00:00Z2024 Sep2026-07-16T21:57:23.047Z2026-07-11T03:08:31.142ZS-EPMC129405093901352610.1016/j.jneumeth.2024.110219