biostudies-literatureMorato-Cecchini CMinisterio de Ciencia, Innovación y UniversidadesGobierno del Principado de AsturiasFundación Caja Rural de Asturias137https://www.ebi.ac.uk/biostudies/studies/S-EPMC12940588biostudies-literatureUnknown12(2)The development of photocurable hydrogel biomaterial inks with suitable rheology, low cytotoxicity, and tuneable mechanical properties is essential for reliable biofabrication. This study aimed to formulate PEGDA-gelatine-collagen inks using lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as photoinitiator. Rheological characterisation and flow-model fitting were performed, mechanical stiffness modulation under different light intensities was evaluated, complex structures were printed using direct extrusion and FRESH methodologies, and PEGDA/LAP extractables were quantified by NMR after controlled washing procedures. In vitro assays assessed cell viability and proliferation on the resulting scaffolds. The Herschel-Bulkley model best described the flow behaviour across formulations; Gels (Basel, Switzerland)Comprehensive Characterisation of Photocurable PEGDA/Gelatine Hydrogels for Extrusion-Based 3D Printing.PMC12940588CER-20231013Beca FCRAPID2022-140635NB-I00IDE/2024/000932Herrada-Manchon HMorato-Cecchini CCelada LFernandez MAAguilar ESanchez-Suarez LRodriguez-Gonzalez DfalseComprehensive Characterisation of Photocurable PEGDA/Gelatine Hydrogels for Extrusion-Based 3D Printing.The development of photocurable hydrogel biomaterial inks with suitable rheology, low cytotoxicity, and tuneable mechanical properties is essential for reliable biofabrication. This study aimed to formulate PEGDA-gelatine-collagen inks using lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as photoinitiator. Rheological characterisation and flow-model fitting were performed, mechanical stiffness modulation under different light intensities was evaluated, complex structures were printed using direct extrusion and FRESH methodologies, and PEGDA/LAP extractables were quantified by NMR after controlled washing procedures. In vitro assays assessed cell viability and proliferation on the resulting scaffolds. The Herschel-Bulkley model best described the flow behaviour across formulations; 2026-01-01T00:00:00Z2026 Feb2026-07-11T03:19:49.922Z2026-07-11T03:12:07.932ZS-EPMC129405884174500910.3390/gels12020137