{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tian D"],"funding":["the National Key Research and Development Programs of China","Shijiazhuang Municipality-Chinese Academy of Agricultural Sciences Cooperative Project","the earmarked fund for Hebei Agriculture Research System","the Precise Nutrition and Healthy Breeding of Donkeys from China Agricultural University","the Innovation Program of the Chinese Academy of Agricultural Science"],"pagination":["80"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12944729"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(2)"],"pubmed_abstract":["A novel, sensitive, and robust LC-MS/MS method was developed and fully validated for the simultaneous determination of fumonisins (FB<sub>1</sub>, FB<sub>2</sub>, FB<sub>3</sub>) and their hydrolyzed metabolites (HFB<sub>1</sub>, HFB<sub>2</sub>, HFB<sub>3</sub>) in donkey plasma, urine, and feces-three critical matrices for toxicokinetic studies. Sample preparation was optimized for each matrix: salting-out assisted liquid-liquid extraction (SALLE) with perchloric acidification for urine and feces, and a dilute-evaporate-shoot (DES) approach for plasma. Chromatographic separation was achieved on a BEH C<sub>18</sub> column with water-ACN containing 0.5% formic acid. The method demonstrated excellent linearity (R<sup>2</sup> ≥ 0.99), acceptable accuracy (mean recoveries: 73.3-111.5%), and "],"journal":["Toxins"],"pubmed_title":["Simultaneous Quantification of Fumonisins and Their Hydrolyzed Metabolites in Donkey Matrices: A Tool for Exposure Assessment and Toxicokinetic Studies."],"pmcid":["PMC12944729"],"funding_grant_id":["2023YFD1301001","HBCT2024260407","DEEJ-ZD-2025-11-299","Feed Quality and Safety","HBCT2024280203","2525001902A"],"pubmed_authors":["Ma Q","Zheng Y","Li Y","Wang P","Wang R","Lin G","Tian D","Zhu R","Xing Q"],"additional_accession":[]},"is_claimable":false,"name":"Simultaneous Quantification of Fumonisins and Their Hydrolyzed Metabolites in Donkey Matrices: A Tool for Exposure Assessment and Toxicokinetic Studies.","description":"A novel, sensitive, and robust LC-MS/MS method was developed and fully validated for the simultaneous determination of fumonisins (FB<sub>1</sub>, FB<sub>2</sub>, FB<sub>3</sub>) and their hydrolyzed metabolites (HFB<sub>1</sub>, HFB<sub>2</sub>, HFB<sub>3</sub>) in donkey plasma, urine, and feces-three critical matrices for toxicokinetic studies. Sample preparation was optimized for each matrix: salting-out assisted liquid-liquid extraction (SALLE) with perchloric acidification for urine and feces, and a dilute-evaporate-shoot (DES) approach for plasma. Chromatographic separation was achieved on a BEH C<sub>18</sub> column with water-ACN containing 0.5% formic acid. The method demonstrated excellent linearity (R<sup>2</sup> ≥ 0.99), acceptable accuracy (mean recoveries: 73.3-111.5%), and ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-11T03:16:34.483Z","creation":"2026-07-11T03:09:04.159Z"},"accession":"S-EPMC12944729","cross_references":{"pubmed":["41745746"],"doi":["10.3390/toxins18020080"]}}