biostudies-literatureTian Dthe National Key Research and Development Programs of ChinaShijiazhuang Municipality-Chinese Academy of Agricultural Sciences Cooperative Projectthe earmarked fund for Hebei Agriculture Research Systemthe Precise Nutrition and Healthy Breeding of Donkeys from China Agricultural Universitythe Innovation Program of the Chinese Academy of Agricultural Science80https://www.ebi.ac.uk/biostudies/studies/S-EPMC12944729biostudies-literatureUnknown18(2)A novel, sensitive, and robust LC-MS/MS method was developed and fully validated for the simultaneous determination of fumonisins (FB₁, FB₂, FB₃) and their hydrolyzed metabolites (HFB₁, HFB₂, HFB₃) in donkey plasma, urine, and feces-three critical matrices for toxicokinetic studies. Sample preparation was optimized for each matrix: salting-out assisted liquid-liquid extraction (SALLE) with perchloric acidification for urine and feces, and a dilute-evaporate-shoot (DES) approach for plasma. Chromatographic separation was achieved on a BEH C₁₈ column with water-ACN containing 0.5% formic acid. The method demonstrated excellent linearity (R² ≥ 0.99), acceptable accuracy (mean recoveries: 73.3-111.5%), and ToxinsSimultaneous Quantification of Fumonisins and Their Hydrolyzed Metabolites in Donkey Matrices: A Tool for Exposure Assessment and Toxicokinetic Studies.PMC129447292023YFD1301001HBCT2024260407DEEJ-ZD-2025-11-299Feed Quality and SafetyHBCT20242802032525001902AMa QZheng YLi YWang PWang RLin GTian DZhu RXing QfalseSimultaneous Quantification of Fumonisins and Their Hydrolyzed Metabolites in Donkey Matrices: A Tool for Exposure Assessment and Toxicokinetic Studies.A novel, sensitive, and robust LC-MS/MS method was developed and fully validated for the simultaneous determination of fumonisins (FB₁, FB₂, FB₃) and their hydrolyzed metabolites (HFB₁, HFB₂, HFB₃) in donkey plasma, urine, and feces-three critical matrices for toxicokinetic studies. Sample preparation was optimized for each matrix: salting-out assisted liquid-liquid extraction (SALLE) with perchloric acidification for urine and feces, and a dilute-evaporate-shoot (DES) approach for plasma. Chromatographic separation was achieved on a BEH C₁₈ column with water-ACN containing 0.5% formic acid. The method demonstrated excellent linearity (R² ≥ 0.99), acceptable accuracy (mean recoveries: 73.3-111.5%), and 2026-01-01T00:00:00Z2026 Feb2026-07-11T03:16:34.483Z2026-07-11T03:09:04.159ZS-EPMC129447294174574610.3390/toxins18020080