{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zambrano Siri RT"],"funding":["Consejo Nacional de Investigaciones Científicas y Técnicas","Canada-Israel Industrial Research and Development Foundation","Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación","Agencia Nacional de Promoción Científica y Tecnológica"],"pagination":["e0343367"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12944795"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(2)"],"pubmed_abstract":["Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, usually known as TriTryps, are the causal agents of animal and human sickness, and are characterized by having complex life cycles, alternating between a mammalian host and an insect vector. Their genes are organized in long transcriptional units that give rise to polycistronic transcripts which maturate into mRNA by a process known as trans-splicing. Among those genes, an important subset is composed of multi-copy genes, which play crucial roles in host invasion and immune evasion. Here, we predicted the most likely trans-splicing acceptor sites (TASs) for TriTryps and found that the average chromatin organization is very similar among them with a mild nucleosome depletion at the TASs, and the same layout is observed in most of the genome. A detailed examination of the nucleosome landscapes resulting from different levels of chromatin digestion in T. brucei shows that an MNase-sensitive complex is protecting the TASs, and it is at least partly composed of histones. Additionally, comparative analysis for single and multi-copy genes in T. cruzi revealed a differential chromatin structure at the TASs suggesting a novel mechanism to guarantee the fidelity of trans-splicing in trypanosomatids."],"journal":["PloS one"],"pubmed_title":["Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids."],"pmcid":["PMC12944795"],"funding_grant_id":["IDRC-Project 109929","PICT Raíces 2019-4260","PICT 2020-00473","PIP-2021-2023-03073","PIBBA 2020-28720210100100CO"],"pubmed_authors":["Beati P","Inchausti L","Smircich P","Alonso GD","Ocampo J","Zambrano Siri RT"],"additional_accession":[]},"is_claimable":false,"name":"Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids.","description":"Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, usually known as TriTryps, are the causal agents of animal and human sickness, and are characterized by having complex life cycles, alternating between a mammalian host and an insect vector. Their genes are organized in long transcriptional units that give rise to polycistronic transcripts which maturate into mRNA by a process known as trans-splicing. Among those genes, an important subset is composed of multi-copy genes, which play crucial roles in host invasion and immune evasion. Here, we predicted the most likely trans-splicing acceptor sites (TASs) for TriTryps and found that the average chromatin organization is very similar among them with a mild nucleosome depletion at the TASs, and the same layout is observed in most of the genome. A detailed examination of the nucleosome landscapes resulting from different levels of chromatin digestion in T. brucei shows that an MNase-sensitive complex is protecting the TASs, and it is at least partly composed of histones. Additionally, comparative analysis for single and multi-copy genes in T. cruzi revealed a differential chromatin structure at the TASs suggesting a novel mechanism to guarantee the fidelity of trans-splicing in trypanosomatids.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026","modification":"2026-07-11T03:18:14.101Z","creation":"2026-07-11T03:12:06.859Z"},"accession":"S-EPMC12944795","cross_references":{"pubmed":["41746921"],"doi":["10.1371/journal.pone.0343367"]}}