biostudies-literatureAshkani EGNCI NIH HHS195https://www.ebi.ac.uk/biostudies/studies/S-EPMC12944968biostudies-literatureUnknown14(2)<h4>Background/objectives</h4>Vaccines targeting melanoma antigens can elicit CD8⁺ T cell responses, but a growing body of work suggests CD4⁺ T cells also play a role in tumor control. Induction of CD4⁺ cells may also support B cells in producing tumor antigen-specific antibodies (Abs). We investigated Abs induced by vaccination with a cocktail of six class II MHC-restricted melanoma peptides (6MHP) and the effect of adjuvant type on Ab isotypes. We hypothesized that the vaccines would induce Abs that respond to different epitopes on individual peptides and that IgG isotype distribution varies with different vaccine adjuvants.<h4>Methods</h4>Sera from patients who received a 6MHP vaccine were evaluated with enzyme-linked immunosorbent assays to map epitopesVaccinesEvaluation of Antibodies Induced by Melanoma Helper Peptide Vaccine and Their Modulation by Vaccine Adjuvants.PMC12944968R21CA105777R01CA178846P30CA044579R03CA219715Ashkani EGTaylor JJOlson WCDickinson AMSlingluff CLfalseEvaluation of Antibodies Induced by Melanoma Helper Peptide Vaccine and Their Modulation by Vaccine Adjuvants.<h4>Background/objectives</h4>Vaccines targeting melanoma antigens can elicit CD8⁺ T cell responses, but a growing body of work suggests CD4⁺ T cells also play a role in tumor control. Induction of CD4⁺ cells may also support B cells in producing tumor antigen-specific antibodies (Abs). We investigated Abs induced by vaccination with a cocktail of six class II MHC-restricted melanoma peptides (6MHP) and the effect of adjuvant type on Ab isotypes. We hypothesized that the vaccines would induce Abs that respond to different epitopes on individual peptides and that IgG isotype distribution varies with different vaccine adjuvants.<h4>Methods</h4>Sera from patients who received a 6MHP vaccine were evaluated with enzyme-linked immunosorbent assays to map epitopes2026-01-01T00:00:00Z2026 Feb2026-07-11T03:17:05.681Z2026-07-11T03:11:51.583ZS-EPMC129449684174611510.3390/vaccines14020195