biostudies-literatureZaitsev AVAmerican Heart AssociationVirginia Polytechnic Institute and State UniversityCollege of Agriculture and Life Sciences, Virginia Polytechnic Institute and State UniversityNIH HHS7705https://www.ebi.ac.uk/biostudies/studies/S-EPMC12946242biostudies-literatureUnknown16(1)Several groups have demonstrated that PERM1 is a positive regulator of mitochondrial bioenergetics in the heart. However, conflicting results have emerged regarding whether PERM1 loss of function affects cardiac contractility. Here, we present data from a retrospective study compiling echocardiography data from all Perm1-knockout (Perm1-KO) mice and their wild-type (WT) littermates used for various molecular biology experiments in our laboratory between April 2022 and September 2023. This yielded an atypically large number of subjects per group-84 WT mice and 88 Perm1-KO mice. We analyzed echocardiography-derived parameters of left ventricular (LV) systolic function. The ejection fraction (EF) was 65.43 ± 7.13% in WT vs. 53.98 ± 8.80% in Perm1-KO mice (p = 5.21E-17, unpaired t-test). OtherScientific reportsAnalysis of systolic cardiac function in PERM1-knockout mice using large cohorts of animals.PMC12946242R01HL156667Virginia Tech Research and Innovation Postdoctoral ScholarshipFBRI Virginia Tech Carilion Operation Fund24POST1186520Warren JSZaitsev AVGoode BSreedevi KfalseAnalysis of systolic cardiac function in PERM1-knockout mice using large cohorts of animals.Several groups have demonstrated that PERM1 is a positive regulator of mitochondrial bioenergetics in the heart. However, conflicting results have emerged regarding whether PERM1 loss of function affects cardiac contractility. Here, we present data from a retrospective study compiling echocardiography data from all Perm1-knockout (Perm1-KO) mice and their wild-type (WT) littermates used for various molecular biology experiments in our laboratory between April 2022 and September 2023. This yielded an atypically large number of subjects per group-84 WT mice and 88 Perm1-KO mice. We analyzed echocardiography-derived parameters of left ventricular (LV) systolic function. The ejection fraction (EF) was 65.43 ± 7.13% in WT vs. 53.98 ± 8.80% in Perm1-KO mice (p = 5.21E-17, unpaired t-test). Other2026-01-01T00:00:00Z2026 Feb2026-07-16T22:21:57.011Z2026-07-13T03:07:25.264ZS-EPMC129462424165459910.1038/s41598-026-37420-2