biostudies-literatureKuran GHealth Sciences University Scientific Research Projects Coordination Unit7666https://www.ebi.ac.uk/biostudies/studies/S-EPMC12946323biostudies-literatureUnknown16(1)Laryngeal cancer has a poor prognosis and invasive character. Despite recent technological advances, laryngeal cancer remains challenging with a low survival rate. Artocarpus lakoocha Roxb. (AL) is rich in oxyresveratrol with antioxidant, cytotoxic, and anti-inflammatory properties This study aimed to determine the total phenolic content, antioxidant capacity and in-vitro anticancer effects of AL on laryngeal cancer cell lines. Total phenolic content and antioxidant activity of AL was evaluated using Folin-Ciocalte and DPPH tests. Cytotoxic and antiproliferative effects were measured with MTT and live-dead assays, while CASP-3, BAX, and BCL-2 expressions were analyzed via qRT-PCR and western blotting. Cell migration inhibition was determined by scratch-wound healing assay. The total phenolScientific reportsThe antioxidant capacity and in-vitro anticancer effects of Artocarpus lakoocha Roxb. against laryngeal cancer cells.PMC129463232022/136Kuran GCanatar IOzdas SOzdas TBaldemir Kilic AfalseThe antioxidant capacity and in-vitro anticancer effects of Artocarpus lakoocha Roxb. against laryngeal cancer cells.Laryngeal cancer has a poor prognosis and invasive character. Despite recent technological advances, laryngeal cancer remains challenging with a low survival rate. Artocarpus lakoocha Roxb. (AL) is rich in oxyresveratrol with antioxidant, cytotoxic, and anti-inflammatory properties This study aimed to determine the total phenolic content, antioxidant capacity and in-vitro anticancer effects of AL on laryngeal cancer cell lines. Total phenolic content and antioxidant activity of AL was evaluated using Folin-Ciocalte and DPPH tests. Cytotoxic and antiproliferative effects were measured with MTT and live-dead assays, while CASP-3, BAX, and BCL-2 expressions were analyzed via qRT-PCR and western blotting. Cell migration inhibition was determined by scratch-wound healing assay. The total phenol2026-01-01T00:00:00Z2026 Feb2026-07-16T22:17:07.422Z2026-07-12T03:08:58.045ZS-EPMC129463234165461410.1038/s41598-026-38501-y