{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao JJ"],"funding":["Sichuan Province Science and Technology Support Program","National Natural Science Foundation of China","Sichuan Provincial Science and Technology Support Program"],"pagination":["1428-1445"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12946459"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(3)"],"pubmed_abstract":["The plant cuticle, composed of cutin and wax, is crucial for reducing non-stomatal water loss and enhancing drought tolerance. CYP86A genes are key regulators of cutin and wax biosynthesis, yet the mechanisms controlling their expression remain unclear. Here, we identified PtrC2H2.2-6, a C2H2-type transcription factor that is downregulated at both transcriptional and protein levels under water stress in poplar. Through bioinformatics and biochemical analyses, we demonstrated that PtrC2H2.2-6 interacts with PtrPPK1 to regulate drought tolerance by modulating two critical cuticle biosynthesis genes, PtrCYP86A7 and PtrCYP86A8. PtrC2H2.2-6 negatively regulates cutin and wax biosynthesis by binding to the CACT motif in the promoters of PtrCYP86A7 and PtrCYP86A8, thereby suppressing their expres"],"journal":["Plant biotechnology journal"],"pubmed_title":["The PtrC2H2.2-6-PtrCYP86A7/A8 Module Regulates Poplar Drought Tolerance Through Mediating Cutin and Wax Biosynthesis Pathways."],"pmcid":["PMC12946459"],"funding_grant_id":["32101481","32471820","2024YFNH0028"],"pubmed_authors":["Yang H","Deng SL","Li H","He F","Li J","Huang J","Wan XQ","Chen LH","Zhao JJ","Zhang Y","Wang T","Yang P","Xiang X","Wang RQ","Wei SY"],"additional_accession":[]},"is_claimable":false,"name":"The PtrC2H2.2-6-PtrCYP86A7/A8 Module Regulates Poplar Drought Tolerance Through Mediating Cutin and Wax Biosynthesis Pathways.","description":"The plant cuticle, composed of cutin and wax, is crucial for reducing non-stomatal water loss and enhancing drought tolerance. CYP86A genes are key regulators of cutin and wax biosynthesis, yet the mechanisms controlling their expression remain unclear. Here, we identified PtrC2H2.2-6, a C2H2-type transcription factor that is downregulated at both transcriptional and protein levels under water stress in poplar. Through bioinformatics and biochemical analyses, we demonstrated that PtrC2H2.2-6 interacts with PtrPPK1 to regulate drought tolerance by modulating two critical cuticle biosynthesis genes, PtrCYP86A7 and PtrCYP86A8. PtrC2H2.2-6 negatively regulates cutin and wax biosynthesis by binding to the CACT motif in the promoters of PtrCYP86A7 and PtrCYP86A8, thereby suppressing their expres","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Mar","modification":"2026-07-16T22:27:05.313Z","creation":"2026-07-11T03:12:17.389Z"},"accession":"S-EPMC12946459","cross_references":{"pubmed":["41133461"],"doi":["10.1111/pbi.70419"]}}