biostudies-literatureBreville GSwiss National Science Foundation261-273https://www.ebi.ac.uk/biostudies/studies/S-EPMC12946607biostudies-literatureUnknown99(1)<h4>Objective</h4>Hepatocyte growth factor (HGF) binds exclusively the c-Met surface receptor, and the HGF/c-Met axis regulates T cell function in autoimmune diseases. We analyzed c-Met expression on human CD4 T cells in the blood and cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) versus non-inflammatory neurological disease (NIND), to better understand the role of CD4 T cells in MS.<h4>Methods</h4>We recruited 34 untreated MS patients (age 28-44 years) and 13 NIND (age 34-51 years) who underwent paired blood and CSF sampling at the time of diagnosis work-up. Phenotypic and functional CD4 T cells characterization was determined by flow cytometry and bulk RNA sequencing. Adhesion and transmigration capacities were studied to further characterize the function of c-Met<sAnnals of neurologyPro-Inflammatory c-Met&lt;sup&gt;+&lt;/sup&gt; CD4 T Cells in Multiple Sclerosis.PMC12946607P500PM_222181310030310030_214931214931Rezk ABenkhoucha MLalive PHSenoner IBreville GBar-Or ATran NLfalsePro-Inflammatory c-Met&lt;sup&gt;+&lt;/sup&gt; CD4 T Cells in Multiple Sclerosis.<h4>Objective</h4>Hepatocyte growth factor (HGF) binds exclusively the c-Met surface receptor, and the HGF/c-Met axis regulates T cell function in autoimmune diseases. We analyzed c-Met expression on human CD4 T cells in the blood and cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) versus non-inflammatory neurological disease (NIND), to better understand the role of CD4 T cells in MS.<h4>Methods</h4>We recruited 34 untreated MS patients (age 28-44 years) and 13 NIND (age 34-51 years) who underwent paired blood and CSF sampling at the time of diagnosis work-up. Phenotypic and functional CD4 T cells characterization was determined by flow cytometry and bulk RNA sequencing. Adhesion and transmigration capacities were studied to further characterize the function of c-Met<s2026-01-01T00:00:00Z2026 Jan2026-07-16T22:27:29.195Z2026-07-11T03:11:58.83ZS-EPMC129466074100210910.1002/ana.78035