biostudies-literatureCheng YNIBIB NIH HHS12https://www.ebi.ac.uk/biostudies/studies/S-EPMC12948959biostudies-literatureUnknown4(1)This study reports the first clinical longitudinal photoacoustic imaging (PAI) of chronic foot ulcers, a major complication in patients with peripheral vascular disorders. Compared to traditional methods such as ABI or near-infrared spectroscopy, the photoacoustic imaging approach provides non-invasive, high-resolution, and quantitative monitoring of vascular dynamics over time. Our system provided dorsal-side imaging of vascular structures with an expanded field of view and incorporated a skin artifact suppression algorithm to improve visualization of subdermal vasculature. From the acquired 2D and 3D images, we extracted a set of 45 quantitative features, representing signal intensity, texture complexity, and morphological changes associated with ulcer progression. Using a LASSO-based feNpj imagingPredictive modeling of chronic foot ulcer outcomes using longitudinal photoacoustic imaging.PMC12948959R01EB035188R01EB028978Bing RWHuang CHarris LMXi YXia JYu SLZhang HZhang XKomornicki IChakraborty SXu WCheng YfalsePredictive modeling of chronic foot ulcer outcomes using longitudinal photoacoustic imaging.This study reports the first clinical longitudinal photoacoustic imaging (PAI) of chronic foot ulcers, a major complication in patients with peripheral vascular disorders. Compared to traditional methods such as ABI or near-infrared spectroscopy, the photoacoustic imaging approach provides non-invasive, high-resolution, and quantitative monitoring of vascular dynamics over time. Our system provided dorsal-side imaging of vascular structures with an expanded field of view and incorporated a skin artifact suppression algorithm to improve visualization of subdermal vasculature. From the acquired 2D and 3D images, we extracted a set of 45 quantitative features, representing signal intensity, texture complexity, and morphological changes associated with ulcer progression. Using a LASSO-based fe2026-01-01T00:00:00Z2026 Feb2026-07-16T23:31:58.111Z2026-07-12T03:11:17.16ZS-EPMC129489594176088310.1038/s44303-026-00143-0