biostudies-literatureCollins ENEI NIH HHSe13321https://www.ebi.ac.uk/biostudies/studies/S-EPMC12949407biostudies-literatureUnknown29(1)<h4>Objective</h4>To investigate the level of pro-inflammatory cytokines in aqueous humor from ADAMTS10-mutant research Beagles at an early stage of open-angle glaucoma (OAG).<h4>Animals studied</h4>Twenty-four research Beagles were enrolled into this case-controlled study. Three groups of ADAMTS10-mutant research Beagles were enrolled in this study: (1) Intraocular pressure (IOP) 15-25 mmHg (normotensive IOP), (2) IOP 25-30 mmHg, and (3) IOP 30-36 mmHg. An unaffected control group of Beagles that were either ADAMTS10-wildtype (WT) or heterozygote-carrier with normotensive IOPs were also enrolled.<h4>Procedures</h4>Aqueous humor samples were collected and analyzed for 16 pro-inflammatory cytokines using the Canine Cytokine SpikeMix and target mass spectrometry via multiple reaction monitorVeterinary ophthalmologyPro-Inflammatory Cytokines in Aqueous Humor From ADAMTS10-Mutant Beagles at an Early Stage of Open-Angle Glaucoma.PMC12949407R01 EY025752R01 EY032478Harman CDCollins ELove KRde Linde Henriksen MMehaffy CKomaromy AMJacobson ALfalsePro-Inflammatory Cytokines in Aqueous Humor From ADAMTS10-Mutant Beagles at an Early Stage of Open-Angle Glaucoma.<h4>Objective</h4>To investigate the level of pro-inflammatory cytokines in aqueous humor from ADAMTS10-mutant research Beagles at an early stage of open-angle glaucoma (OAG).<h4>Animals studied</h4>Twenty-four research Beagles were enrolled into this case-controlled study. Three groups of ADAMTS10-mutant research Beagles were enrolled in this study: (1) Intraocular pressure (IOP) 15-25 mmHg (normotensive IOP), (2) IOP 25-30 mmHg, and (3) IOP 30-36 mmHg. An unaffected control group of Beagles that were either ADAMTS10-wildtype (WT) or heterozygote-carrier with normotensive IOPs were also enrolled.<h4>Procedures</h4>Aqueous humor samples were collected and analyzed for 16 pro-inflammatory cytokines using the Canine Cytokine SpikeMix and target mass spectrometry via multiple reaction monitor2026-01-01T00:00:00Z2026 Jan2026-07-16T23:17:49.576Z2026-07-12T03:11:26.127ZS-EPMC129494073994904710.1111/vop.13321