biostudies-literatureUnknown22(8)Fernandez-Medarde AThe mammalian Grf1 and Grf2 proteins are Ras guanine nucleotide exchange factors (GEFs) sharing a high degree of structural homology, as well as an elevated expression level in central nervous system tissues. Such similarities raise questions concerning the specificity and/or redundancy at the functional level between the two Grf proteins. grf1-null mutant mice have been recently described which showed phenotypic growth reduction and long-term memory loss. To gain insight into the in vivo function of Grf2, we disrupted its catalytic CDC25-H domain by means of gene targeting. Breeding among grf2(+/-) animals gave rise to viable grf2(-/-) adult animals with a normal Mendelian pattern, suggesting that Grf2 is not essential for embryonic and adult mouse development. In contrast to Grf1-null miMolecular and cellular biology2498-504https://www.ebi.ac.uk/biostudies/studies/S-EPMC133706biostudies-literatureTargeted disruption of Ras-Grf2 shows its dispensability for mouse growth and development.PMC133706Santos ENunez ATessarollo LEsteban LMFernandez-Medarde APorteros AfalseTargeted disruption of Ras-Grf2 shows its dispensability for mouse growth and development.The mammalian Grf1 and Grf2 proteins are Ras guanine nucleotide exchange factors (GEFs) sharing a high degree of structural homology, as well as an elevated expression level in central nervous system tissues. Such similarities raise questions concerning the specificity and/or redundancy at the functional level between the two Grf proteins. grf1-null mutant mice have been recently described which showed phenotypic growth reduction and long-term memory loss. To gain insight into the in vivo function of Grf2, we disrupted its catalytic CDC25-H domain by means of gene targeting. Breeding among grf2(+/-) animals gave rise to viable grf2(-/-) adult animals with a normal Mendelian pattern, suggesting that Grf2 is not essential for embryonic and adult mouse development. In contrast to Grf1-null mi2002-01-01T00:00:00Z2002 Apr2025-04-04T12:41:30.965Z2019-03-27T00:17:21ZS-EPMC1337061190994410.1128/mcb.22.8.2498-2504.200210.1128/MCB.22.8.2498-2504.2002