biostudies-literatureUnknown34(3)Wiesenthal VRegulation of translation is critical for the accurate expression of a broad variety of genes that function in cell cycle progression and cell differentiation, as well as in the adaptation to cellular stress. The aetiologies of a number of human diseases, including cancer, have been linked to mutations in genes that control mRNA translation, or in cis-regulatory mRNA-sequences. Therefore, research on translational control and its therapeutic appliance has become most important. However, to date only a limited number of therapeutic drugs are known to affect translational control. Here we describe a novel, straightforward approach for the detection of cellular translational activity. We developed a Translational Control Reporter System (TCRS), which utilizes the cis-regulatory upstream open Nucleic acids researche23https://www.ebi.ac.uk/biostudies/studies/S-EPMC1363784biostudies-literatureA translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell.PMC1363784Leutz AWiesenthal VCalkhoven CFfalseA translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell.Regulation of translation is critical for the accurate expression of a broad variety of genes that function in cell cycle progression and cell differentiation, as well as in the adaptation to cellular stress. The aetiologies of a number of human diseases, including cancer, have been linked to mutations in genes that control mRNA translation, or in cis-regulatory mRNA-sequences. Therefore, research on translational control and its therapeutic appliance has become most important. However, to date only a limited number of therapeutic drugs are known to affect translational control. Here we describe a novel, straightforward approach for the detection of cellular translational activity. We developed a Translational Control Reporter System (TCRS), which utilizes the cis-regulatory upstream open 2006-01-01T00:00:00Z2006 Feb2025-04-19T20:53:47.416Z2019-03-27T01:25:53ZS-EPMC13637841647384610.1093/nar/gnj029