{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiao Z"],"funding":["NHLBI NIH HHS"],"pagination":["2221-34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1386801"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["90(6)"],"pubmed_abstract":["Platelet-leukocyte adhesion may contribute to thrombosis and inflammation. We examined the heterotypic interaction between unactivated neutrophils and either thrombin receptor activating peptide (TRAP)-stimulated platelets or P-selectin-bearing beads (Ps-beads) in suspension. Cone-plate viscometers were used to apply controlled shear rates from 14 to 3000/s. Platelet-neutrophil and bead-neutrophil adhesion analysis was performed using both flow cytometry and high-speed videomicroscopy. We observed that although blocking antibodies against either P-selectin or P-selectin glycoprotein ligand-1 (PSGL-1) alone inhibited platelet-neutrophil adhesion by approximately 60% at 140/s, these reagents completely blocked adhesion at 3000/s. Anti-Mac-1 alone did not alter platelet-neutrophil adhesion ra"],"journal":["Biophysical journal"],"pubmed_title":["Biomechanics of P-selectin PSGL-1 bonds: shear threshold and integrin-independent cell adhesion."],"pmcid":["PMC1386801"],"funding_grant_id":["K02 HL076211","HL63014","R01 HL063014","HL76211"],"pubmed_authors":["Goldsmith HL","Xiao Z","McIntosh FA","Neelamegham S","Shankaran H"],"additional_accession":[]},"is_claimable":false,"name":"Biomechanics of P-selectin PSGL-1 bonds: shear threshold and integrin-independent cell adhesion.","description":"Platelet-leukocyte adhesion may contribute to thrombosis and inflammation. We examined the heterotypic interaction between unactivated neutrophils and either thrombin receptor activating peptide (TRAP)-stimulated platelets or P-selectin-bearing beads (Ps-beads) in suspension. Cone-plate viscometers were used to apply controlled shear rates from 14 to 3000/s. Platelet-neutrophil and bead-neutrophil adhesion analysis was performed using both flow cytometry and high-speed videomicroscopy. We observed that although blocking antibodies against either P-selectin or P-selectin glycoprotein ligand-1 (PSGL-1) alone inhibited platelet-neutrophil adhesion by approximately 60% at 140/s, these reagents completely blocked adhesion at 3000/s. Anti-Mac-1 alone did not alter platelet-neutrophil adhesion ra","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Mar","modification":"2025-04-04T13:41:42.492Z","creation":"2019-03-27T01:26:04Z"},"accession":"S-EPMC1386801","cross_references":{"pubmed":["16387772"],"doi":["10.1529/biophysj.105.065789"]}}