biostudies-literatureXiao ZNHLBI NIH HHS2221-34https://www.ebi.ac.uk/biostudies/studies/S-EPMC1386801biostudies-literatureUnknown90(6)Platelet-leukocyte adhesion may contribute to thrombosis and inflammation. We examined the heterotypic interaction between unactivated neutrophils and either thrombin receptor activating peptide (TRAP)-stimulated platelets or P-selectin-bearing beads (Ps-beads) in suspension. Cone-plate viscometers were used to apply controlled shear rates from 14 to 3000/s. Platelet-neutrophil and bead-neutrophil adhesion analysis was performed using both flow cytometry and high-speed videomicroscopy. We observed that although blocking antibodies against either P-selectin or P-selectin glycoprotein ligand-1 (PSGL-1) alone inhibited platelet-neutrophil adhesion by approximately 60% at 140/s, these reagents completely blocked adhesion at 3000/s. Anti-Mac-1 alone did not alter platelet-neutrophil adhesion raBiophysical journalBiomechanics of P-selectin PSGL-1 bonds: shear threshold and integrin-independent cell adhesion.PMC1386801K02 HL076211HL63014R01 HL063014HL76211Goldsmith HLXiao ZMcIntosh FANeelamegham SShankaran HfalseBiomechanics of P-selectin PSGL-1 bonds: shear threshold and integrin-independent cell adhesion.Platelet-leukocyte adhesion may contribute to thrombosis and inflammation. We examined the heterotypic interaction between unactivated neutrophils and either thrombin receptor activating peptide (TRAP)-stimulated platelets or P-selectin-bearing beads (Ps-beads) in suspension. Cone-plate viscometers were used to apply controlled shear rates from 14 to 3000/s. Platelet-neutrophil and bead-neutrophil adhesion analysis was performed using both flow cytometry and high-speed videomicroscopy. We observed that although blocking antibodies against either P-selectin or P-selectin glycoprotein ligand-1 (PSGL-1) alone inhibited platelet-neutrophil adhesion by approximately 60% at 140/s, these reagents completely blocked adhesion at 3000/s. Anti-Mac-1 alone did not alter platelet-neutrophil adhesion ra2006-01-01T00:00:00Z2006 Mar2025-04-04T13:41:42.492Z2019-03-27T01:26:04ZS-EPMC13868011638777210.1529/biophysj.105.065789