{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sturman DA"],"funding":["NHLBI NIH HHS","NINDS NIH HHS"],"pagination":["L45-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1386803"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["90(6)"],"pubmed_abstract":["The acidity of mammalian secretory vesicles drives concentration and processing of their contents. Here, pH-sensitive green fluorescent protein (GFP) variants show that the > or =30-fold (H+) difference between secretory vesicles (pH < or = 5.7) and the cytoplasm (pH = 7.2) in mammalian cells is not present in peptidergic and small synaptic vesicles of the Drosophila neuromuscular junction. First, we find that fluorescence from Topaz-tagged atrial natriuretic factor, a peptidergic vesicle pH indicator, is only modestly affected by collapsing the H+ gradient in type III synaptic boutons. Quantitation shows that peptidergic vesicles are nearly neutral (pH = 6.74 +/- 0.05), even when temperature is elevated. Furthermore, small synaptic vesicles in glutamatergic synaptic boutons, studied with "],"journal":["Biophysical journal"],"pubmed_title":["Nearly neutral secretory vesicles in Drosophila nerve terminals."],"pmcid":["PMC1386803"],"funding_grant_id":["NS32385","R01 NS032385","R01 HL080632"],"pubmed_authors":["Shakiryanova D","Hewes RS","Levitan ES","Sturman DA","Deitcher DL"],"additional_accession":[]},"is_claimable":false,"name":"Nearly neutral secretory vesicles in Drosophila nerve terminals.","description":"The acidity of mammalian secretory vesicles drives concentration and processing of their contents. Here, pH-sensitive green fluorescent protein (GFP) variants show that the > or =30-fold (H+) difference between secretory vesicles (pH < or = 5.7) and the cytoplasm (pH = 7.2) in mammalian cells is not present in peptidergic and small synaptic vesicles of the Drosophila neuromuscular junction. First, we find that fluorescence from Topaz-tagged atrial natriuretic factor, a peptidergic vesicle pH indicator, is only modestly affected by collapsing the H+ gradient in type III synaptic boutons. Quantitation shows that peptidergic vesicles are nearly neutral (pH = 6.74 +/- 0.05), even when temperature is elevated. Furthermore, small synaptic vesicles in glutamatergic synaptic boutons, studied with ","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Mar","modification":"2025-04-04T13:41:42.779Z","creation":"2019-03-27T01:26:03Z"},"accession":"S-EPMC1386803","cross_references":{"pubmed":["16428282"],"doi":["10.1529/biophysj.106.080978"]}}