{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2"],"submitter":["Van Criekinge W"],"pubmed_abstract":["Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a \"differential\" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to \"real\" antibodies. With the technology available, the"],"journal":["Biological procedures online"],"pagination":["1-38"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC140126"],"repository":["biostudies-literature"],"pubmed_title":["Yeast Two-Hybrid: State of the Art."],"pmcid":["PMC140126"],"pubmed_authors":["Van Criekinge W","Beyaert R"],"additional_accession":[]},"is_claimable":false,"name":"Yeast Two-Hybrid: State of the Art.","description":"Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a \"differential\" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to \"real\" antibodies. With the technology available, the","dates":{"release":"1999-01-01T00:00:00Z","publication":"1999 Oct","modification":"2025-04-05T11:09:14.109Z","creation":"2020-11-07T09:51:22Z"},"accession":"S-EPMC140126","cross_references":{"pubmed":["12734586"],"doi":["10.1251/bpo16"]}}