biostudies-literatureUnknown2Van Criekinge WGenome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, theBiological procedures online1-38https://www.ebi.ac.uk/biostudies/studies/S-EPMC140126biostudies-literatureYeast Two-Hybrid: State of the Art.PMC140126Van Criekinge WBeyaert RfalseYeast Two-Hybrid: State of the Art.Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the1999-01-01T00:00:00Z1999 Oct2025-04-05T11:09:14.109Z2020-11-07T09:51:22ZS-EPMC1401261273458610.1251/bpo16