{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["77(1)"],"submitter":["Vilen H"],"pubmed_abstract":["Advances in DNA transposition technology have recently generated efficient tools for various types of functional genetic analyses. We demonstrate here the power of the bacteriophage Mu-derived in vitro DNA transposition system for modification and functional characterization of a complete bacterial virus genome. The linear double-stranded DNA genome of Escherichia coli bacteriophage PRD1 was studied by insertion mutagenesis with reporter mini-Mu transposons that were integrated in vitro into isolated genomic DNA. After introduction into bacterial cells by electroporation, recombinant transposon-containing virus clones were identified by autoradiography or visual blue-white screening employing alpha-complementation of E. coli beta-galactosidase. Additionally, a modified transposon with engi"],"journal":["Journal of virology"],"pagination":["123-34"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC140628"],"repository":["biostudies-literature"],"pubmed_title":["A direct transposon insertion tool for modification and functional analysis of viral genomes."],"pmcid":["PMC140628"],"pubmed_authors":["Aalto JM","Kassinen A","Savilahti H","Vilen H","Paulin L"],"additional_accession":[]},"is_claimable":false,"name":"A direct transposon insertion tool for modification and functional analysis of viral genomes.","description":"Advances in DNA transposition technology have recently generated efficient tools for various types of functional genetic analyses. We demonstrate here the power of the bacteriophage Mu-derived in vitro DNA transposition system for modification and functional characterization of a complete bacterial virus genome. The linear double-stranded DNA genome of Escherichia coli bacteriophage PRD1 was studied by insertion mutagenesis with reporter mini-Mu transposons that were integrated in vitro into isolated genomic DNA. After introduction into bacterial cells by electroporation, recombinant transposon-containing virus clones were identified by autoradiography or visual blue-white screening employing alpha-complementation of E. coli beta-galactosidase. Additionally, a modified transposon with engi","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Jan","modification":"2025-04-04T14:48:35.737Z","creation":"2019-03-27T00:17:30Z"},"accession":"S-EPMC140628","cross_references":{"pubmed":["12477817"],"doi":["10.1128/jvi.77.1.123-134.2003"]}}