biostudies-literatureTagoh HMedical Research Council1070-80https://www.ebi.ac.uk/biostudies/studies/S-EPMC1409732biostudies-literatureUnknown25(5)The transcription factor Pax5 (BSAP) is required for the expression of a B-cell-specific genetic program and for B-cell differentiation, and also to suppress genes of alternative lineages. The molecular mechanism by which repression of myeloid genes occurs during early B-lineage restriction is unknown and in this study we addressed this question. One of the genes repressed by Pax5 in B cells is the colony-stimulating factor receptor 1 gene (csf1r or c-fms). We examined the changes in chromatin caused by Pax5 activity, and we show that Pax5 is directly recruited to c-fms resulting in the rapid loss of RNA polymerase II binding, followed by loss of transcription factor binding and DNaseI hypersensitivity at all cis-regulatory elements. We also show that Pax5 targets the basal transcription mThe EMBO journalThe mechanism of repression of the myeloid-specific c-fms gene by Pax5 during B lineage restriction.PMC1409732MC_U105161083Bonifer CWarren AJIngram RClarke DBusslinger MSalvagiotto GTagoh HWilson NfalseThe mechanism of repression of the myeloid-specific c-fms gene by Pax5 during B lineage restriction.The transcription factor Pax5 (BSAP) is required for the expression of a B-cell-specific genetic program and for B-cell differentiation, and also to suppress genes of alternative lineages. The molecular mechanism by which repression of myeloid genes occurs during early B-lineage restriction is unknown and in this study we addressed this question. One of the genes repressed by Pax5 in B cells is the colony-stimulating factor receptor 1 gene (csf1r or c-fms). We examined the changes in chromatin caused by Pax5 activity, and we show that Pax5 is directly recruited to c-fms resulting in the rapid loss of RNA polymerase II binding, followed by loss of transcription factor binding and DNaseI hypersensitivity at all cis-regulatory elements. We also show that Pax5 targets the basal transcription m2006-01-01T00:00:00Z2006 Mar2026-05-02T09:06:13.126Z2026-04-07T17:46:50.647ZS-EPMC14097321648221910.1038/sj.emboj.7600997