{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(1)"],"submitter":["Pinzon-Charry A"],"pubmed_abstract":["<h4>Introduction</h4>Dendritic cells (DCs) are key antigen-presenting cells that play an essential role in initiating and directing cellular and humoral immunity, including anti-tumor responses. Due to their critical role in cancer, induction of DC apoptosis may be one of the central mechanisms used by tumors to evade immune recognition.<h4>Methods</h4>Spontaneous apoptosis of blood DCs (lineage negative HLA-DR positive cells) was assessed in peripheral blood mononuclear cells (PBMCs) using Annexin-V and TUNEL assays immediately after blood collection. The role of tumor products was assessed by culturing cells with supernatants derived from breast cancer cell lines (TDSN) or PBMCs (PBMC-SN, as a control). The capacity of DC stimulation to prevent apoptosis was assessed by incubating DC wit"],"journal":["Breast cancer research : BCR"],"pagination":["R5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1413992"],"repository":["biostudies-literature"],"pubmed_title":["Spontaneous apoptosis of blood dendritic cells in patients with breast cancer."],"pmcid":["PMC1413992"],"pubmed_authors":["Pinzon-Charry A","Maxwell T","Schmidt C","Lopez JA","McGuckin MA","Furnival C"],"additional_accession":[]},"is_claimable":false,"name":"Spontaneous apoptosis of blood dendritic cells in patients with breast cancer.","description":"<h4>Introduction</h4>Dendritic cells (DCs) are key antigen-presenting cells that play an essential role in initiating and directing cellular and humoral immunity, including anti-tumor responses. Due to their critical role in cancer, induction of DC apoptosis may be one of the central mechanisms used by tumors to evade immune recognition.<h4>Methods</h4>Spontaneous apoptosis of blood DCs (lineage negative HLA-DR positive cells) was assessed in peripheral blood mononuclear cells (PBMCs) using Annexin-V and TUNEL assays immediately after blood collection. The role of tumor products was assessed by culturing cells with supernatants derived from breast cancer cell lines (TDSN) or PBMCs (PBMC-SN, as a control). The capacity of DC stimulation to prevent apoptosis was assessed by incubating DC wit","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006","modification":"2025-05-29T21:28:21.564Z","creation":"2025-05-29T21:28:21.564Z"},"accession":"S-EPMC1413992","cross_references":{"pubmed":["16417648"],"doi":["10.1186/bcr1361"]}}