biostudies-literatureZeng LNINDS NIH HHSNIGMS NIH HHS10-1https://www.ebi.ac.uk/biostudies/studies/S-EPMC1414792biostudies-literatureUnknown128(1)We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.Journal of the American Chemical SocietyA selective turn-on fluorescent sensor for imaging copper in living cells.PMC1414792T32 GM 066698T32 GM066698R01 NS035549Isacoff EYZeng LChang CJMiller EWPralle AfalseA selective turn-on fluorescent sensor for imaging copper in living cells.We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.2006-01-01T00:00:00Z2006 Jan2024-11-05T22:11:53.855Z2019-03-27T01:26:09ZS-EPMC14147921639009610.1021/ja055064u