{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["72(4)"],"submitter":["Providenti MA"],"pubmed_abstract":["In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture method, cloned, and sequenced. The 3Nba dioxygenase (MnbA) is a member of the phthalate family of aromatic oxygenases. An open reading frame designated mnbB that codes for an NAD(P)H-dependent class IA aromatic oxidoreductase is downstream of mnbA. MnbB is tentatively identified as the oxidoreductase that transfers reducing equivalents to MnbA in strain JS46. The mnb locus is flanked by IS1071 elements. The upstream element is interrupted by a novel insertion sequence designat"],"journal":["Applied and environmental microbiology"],"pagination":["2651-60"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1449074"],"repository":["biostudies-literature"],"pubmed_title":["The locus coding for the 3-nitrobenzoate dioxygenase of Comamonas sp. strain JS46 is flanked by IS1071 elements and is subject to deletion and inversion events."],"pmcid":["PMC1449074"],"pubmed_authors":["O'brien JM","Providenti MA","Rosolen S","Wyndham RC","Shaye RE","Lynes KD","McKenna NT","Lambert IB"],"additional_accession":[]},"is_claimable":false,"name":"The locus coding for the 3-nitrobenzoate dioxygenase of Comamonas sp. strain JS46 is flanked by IS1071 elements and is subject to deletion and inversion events.","description":"In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture method, cloned, and sequenced. The 3Nba dioxygenase (MnbA) is a member of the phthalate family of aromatic oxygenases. An open reading frame designated mnbB that codes for an NAD(P)H-dependent class IA aromatic oxidoreductase is downstream of mnbA. MnbB is tentatively identified as the oxidoreductase that transfers reducing equivalents to MnbA in strain JS46. The mnb locus is flanked by IS1071 elements. The upstream element is interrupted by a novel insertion sequence designat","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Apr","modification":"2025-04-18T18:27:51.21Z","creation":"2019-03-27T01:26:19Z"},"accession":"S-EPMC1449074","cross_references":{"pubmed":["16597970"],"doi":["10.1128/AEM.72.4.2651-2660.2006","10.1128/aem.72.4.2651-2660.2006"]}}