{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cummins JM"],"funding":["NCI NIH HHS"],"pagination":["3687-92"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1450142"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["103(10)"],"pubmed_abstract":["MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer "],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["The colorectal microRNAome."],"pmcid":["PMC1450142"],"funding_grant_id":["CA057345","R37 CA057345","R01 CA057345"],"pubmed_authors":["Pagliarini R","Kinzler KW","Raymond CK","Velculescu VE","Barad O","Cummins JM","Bentwich Z","He Y","Diaz LA","Sjoblom T","Vogelstein B","Labourier E","Roberts BS","Leary RJ","Szafranska AE","Juhl H"],"additional_accession":[]},"is_claimable":false,"name":"The colorectal microRNAome.","description":"MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer ","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Mar","modification":"2025-04-19T15:47:13.039Z","creation":"2019-03-27T01:26:20Z"},"accession":"S-EPMC1450142","cross_references":{"pubmed":["16505370"],"doi":["10.1073/pnas.0511155103"]}}