{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ivens AC"],"funding":["NIAID NIH HHS","Wellcome Trust"],"pagination":["436-42"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1470643"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["309(5733)"],"pubmed_abstract":["Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression."],"journal":["Science (New York, N.Y.)"],"pubmed_title":["The genome of the kinetoplastid parasite, Leishmania major."],"pmcid":["PMC1470643"],"funding_grant_id":["R01 AI040599","R01 AI053667"],"pubmed_authors":["Pentony M","Cadag E","Purnelle B","Schwartz DC","Volckaert G","Hertz-Fowler C","Pohl TM","Coulson RM","Duesterhoeft A","Smith DF","Tosato V","Cruz AK","Vogt C","Blackwell JM","Matthews K","Seeger K","Schafer M","Horn D","Bason N","Beck A","Klages S","Zimmermann W","Bauser C","Stuart KD","Squares R","Squares S","Rabbinowitsch E","Frasch AC","Gabel C","Beverley SM","Davies RM","Bothe G","Ivens AC","Goffeau A","Munden H","Larke N","Ruiz JC","Seyler A","Hilbert H","Woodward J","Bruschi CV","Zhou S","Norbertczak H","Sisk E","Borzym K","Sivam D","Goble A","Huang Y","Wambutt R","Apostolou Z","Price C","O'neil S","Warren T","Peacock CS","Fraser A","Shin H","Rieger M","Mottram JC","Saunders D","Muller-Auer S","Aert R","Litvin L","Marra M","Ciarloni L","Rutter S","Kube M","Worthey EA","Attipoe P","Rajandream MA","Cronin A","Fosker N","Wedler H","Aggarwal G","Reinhardt R","Fazelina G","Robben J","Nelson S","Myler PJ","Schein J","Quail MA","Barrell B","Murphy L","Sharp S","De Gaudenzi J","Masuy D","Dobson DE","Clayton C","Louie T","Knights A","Adlem E","Anupama A","Fuchs M","Berriman M","Harris D","Lord A","Michaeli S","Oliver K","Collins M","Rinta J","Bianchettin G","Robertson L"],"additional_accession":[]},"is_claimable":false,"name":"The genome of the kinetoplastid parasite, Leishmania major.","description":"Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Jul","modification":"2020-10-29T12:44:05Z","creation":"2019-03-27T01:45:24Z"},"accession":"S-EPMC1470643","cross_references":{"pubmed":["16020728"],"doi":["10.1126/science.1112680"]}}