{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sharp DJ"],"funding":["NIGMS NIH HHS"],"pagination":["241-53"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC14771"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear. Here we visualize spindle formation in living Drosophila embryos to show that spindle pole movements are directed by a temporally coordinated balance of forces generated by three mitotic motors, cytoplasmic dynein, KLP61F, and Ncd. Specifically, our findings suggest that dynein acts to move the poles apart throughout mitosis and that this activity is augmented by KLP61F after the fenestration of the nuclear envelope, a process analogous to nuclear envelope breakdown, which occurs at the onset of prometaphase. Conversely, we find that Ncd generates forces that pull the poles together between interph"],"journal":["Molecular biology of the cell"],"pubmed_title":["Functional coordination of three mitotic motors in Drosophila embryos."],"pmcid":["PMC14771"],"funding_grant_id":["GM-55507","GM-19262","F32 GM019262","R01 GM055507"],"pubmed_authors":["Brown HM","Kwon M","Rogers GC","Sharp DJ","Scholey JM","Holland G"],"additional_accession":[]},"is_claimable":false,"name":"Functional coordination of three mitotic motors in Drosophila embryos.","description":"It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear. Here we visualize spindle formation in living Drosophila embryos to show that spindle pole movements are directed by a temporally coordinated balance of forces generated by three mitotic motors, cytoplasmic dynein, KLP61F, and Ncd. Specifically, our findings suggest that dynein acts to move the poles apart throughout mitosis and that this activity is augmented by KLP61F after the fenestration of the nuclear envelope, a process analogous to nuclear envelope breakdown, which occurs at the onset of prometaphase. Conversely, we find that Ncd generates forces that pull the poles together between interph","dates":{"release":"2000-01-01T00:00:00Z","publication":"2000 Jan","modification":"2025-04-21T16:31:31.878Z","creation":"2019-03-26T23:42:37Z"},"accession":"S-EPMC14771","cross_references":{"pubmed":["10637305"],"doi":["10.1091/mbc.11.1.241"]}}