{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Duan X"],"funding":["NIDDK NIH HHS","PHS HHS"],"pagination":["149-58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1479762"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["397(1)"],"pubmed_abstract":["mRNA profiling has been extensively used to study muscle wasting. mRNA level changes may not reflect that of proteins, especially in catabolic muscle where there is decreased synthesis and increased degradation. As sepsis is often associated with burn injury, and burn superimposed by sepsis has been shown to result in significant loss of lean tissues, we characterized changes in the skeletal-muscle proteome of rats subjected to a cutaneous burn covering 20% of the total body surface area, followed 2 days later by sepsis induced by CLP (caecal ligation and puncture). EDL (extensor digitorum longus) muscles were dissected from Burn-CLP animals (n=4) and controls (sham-burned and sham-CLP-treated, n=4). Burn-CLP injury resulted in a rapid loss of EDL weight, increased ubiquitin-conjugated pro"],"journal":["The Biochemical journal"],"pubmed_title":["Proteomic analysis of altered protein expression in skeletal muscle of rats in a hypermetabolic state induced by burn sepsis."],"pmcid":["PMC1479762"],"funding_grant_id":["P30 DK040561-11","P30 DK040561","R01 A1063795"],"pubmed_authors":["Yarmush D","Yarmush ML","Berthiaume F","Duan X"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic analysis of altered protein expression in skeletal muscle of rats in a hypermetabolic state induced by burn sepsis.","description":"mRNA profiling has been extensively used to study muscle wasting. mRNA level changes may not reflect that of proteins, especially in catabolic muscle where there is decreased synthesis and increased degradation. As sepsis is often associated with burn injury, and burn superimposed by sepsis has been shown to result in significant loss of lean tissues, we characterized changes in the skeletal-muscle proteome of rats subjected to a cutaneous burn covering 20% of the total body surface area, followed 2 days later by sepsis induced by CLP (caecal ligation and puncture). EDL (extensor digitorum longus) muscles were dissected from Burn-CLP animals (n=4) and controls (sham-burned and sham-CLP-treated, n=4). Burn-CLP injury resulted in a rapid loss of EDL weight, increased ubiquitin-conjugated pro","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Jul","modification":"2025-04-05T08:50:25.49Z","creation":"2019-08-03T07:06:26Z"},"accession":"S-EPMC1479762","cross_references":{"pubmed":["16483253"],"doi":["10.1042/bj20051710","10.1042/BJ20051710"]}}