{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["103(25)"],"submitter":["Sernee MF"],"pubmed_abstract":["Infective stages of the protozoan parasite Leishmania spp. accumulate a class of beta-1,2-mannan oligosaccharides as their major carbohydrate reserve material. Here, we describe the biosynthesis of Leishmania mannan. Mannan precursors were identified by metabolic labeling of Leishmania mexicana promastigotes with [(3)H]mannose. Label was initially incorporated into a phosphomannose primer and short phosphorylated beta-1,2-mannan oligomers that were two to five residues long. Analysis of the mannan primer by Fourier transform ion-cyclotron resonance MS and various enzymatic and chemical treatments and comparison with authentic mannose (Man) phosphates indicated the presence of Man-alpha-1,4-cyclic phosphate. This primer was synthesized from Man-6-phosphate by means of Man-1-phosphate in a c"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["9458-63"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1480429"],"repository":["biostudies-literature"],"pubmed_title":["Leishmania beta-1,2-mannan is assembled on a mannose-cyclic phosphate primer."],"pmcid":["PMC1480429"],"pubmed_authors":["Sernee MF","Dinev Z","Williams SJ","Ralton JE","O'Hair RA","McConville MJ","Khairallah GN"],"additional_accession":[]},"is_claimable":false,"name":"Leishmania beta-1,2-mannan is assembled on a mannose-cyclic phosphate primer.","description":"Infective stages of the protozoan parasite Leishmania spp. accumulate a class of beta-1,2-mannan oligosaccharides as their major carbohydrate reserve material. Here, we describe the biosynthesis of Leishmania mannan. Mannan precursors were identified by metabolic labeling of Leishmania mexicana promastigotes with [(3)H]mannose. Label was initially incorporated into a phosphomannose primer and short phosphorylated beta-1,2-mannan oligomers that were two to five residues long. Analysis of the mannan primer by Fourier transform ion-cyclotron resonance MS and various enzymatic and chemical treatments and comparison with authentic mannose (Man) phosphates indicated the presence of Man-alpha-1,4-cyclic phosphate. This primer was synthesized from Man-6-phosphate by means of Man-1-phosphate in a c","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Jun","modification":"2025-04-19T08:42:28.234Z","creation":"2019-03-27T01:45:29Z"},"accession":"S-EPMC1480429","cross_references":{"pubmed":["16766650"],"doi":["10.1073/pnas.0603539103"]}}