{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Winters BS"],"funding":["NIGMS NIH HHS"],"pagination":["3386-96"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1525224"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(8)"],"pubmed_abstract":["Oncogenic transformation has been associated with decreased fibronectin (FN) matrix assembly. For example, both the HT-1080 fibrosarcoma and MAT-LyLu cell lines fail to assemble a FN matrix when grown in monolayer culture (2-dimensional [2D] system). In this study, we show that these cells regain the ability to assemble a FN matrix when they are grown as aggregates (3-dimensional [3D] system). FN matrix assembly in 3D correlates with decreased Raf-1 protein expression compared with cells grown in monolayer culture. This effect is associated with reduced Raf-1 mRNA levels as determined by quantitative RT-PCR and not proteasome-mediated degradation of endogenous Raf-1. Interestingly, transient expression of a Raf-1 promoter-reporter construct demonstrates increased Raf-1 promoter activity in"],"journal":["Molecular biology of the cell"],"pubmed_title":["Three-dimensional culture regulates Raf-1 expression to modulate fibronectin matrix assembly."],"pmcid":["PMC1525224"],"funding_grant_id":["GM-061487"],"pubmed_authors":["Robinson EE","Foty RA","Raj BK","Corbett SA","Winters BS"],"additional_accession":[]},"is_claimable":false,"name":"Three-dimensional culture regulates Raf-1 expression to modulate fibronectin matrix assembly.","description":"Oncogenic transformation has been associated with decreased fibronectin (FN) matrix assembly. For example, both the HT-1080 fibrosarcoma and MAT-LyLu cell lines fail to assemble a FN matrix when grown in monolayer culture (2-dimensional [2D] system). In this study, we show that these cells regain the ability to assemble a FN matrix when they are grown as aggregates (3-dimensional [3D] system). FN matrix assembly in 3D correlates with decreased Raf-1 protein expression compared with cells grown in monolayer culture. This effect is associated with reduced Raf-1 mRNA levels as determined by quantitative RT-PCR and not proteasome-mediated degradation of endogenous Raf-1. Interestingly, transient expression of a Raf-1 promoter-reporter construct demonstrates increased Raf-1 promoter activity in","dates":{"release":"2006-01-01T00:00:00Z","publication":"2006 Aug","modification":"2025-04-21T18:14:26.237Z","creation":"2019-03-27T01:45:41Z"},"accession":"S-EPMC1525224","cross_references":{"pubmed":["16707572"],"doi":["10.1091/mbc.e05-09-0849"]}}