{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Binley JM"],"funding":["NIAID NIH HHS"],"pagination":["5678-84"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC154040"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["77(10)"],"pubmed_abstract":["We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M. Binley, R. W. Sanders, B. Clas, N. Schuelke, A. Master, Y. Guo, F. Kajumo, D. J. Anselma, P. J. Maddon, W. C. Olson, and J. P. Moore, J. Virol. 74:627-643, 2000). Here we produced pseudovirions bearing the mutant envelope and a reporter gene to examine the mutant's infectious properties. These pseudovirions attach to cells expressing CD4 and coreceptor but infect only when triggered with reducing agent, implying that gp120-gp41 dissociation is necessary for infection. Further studies suggested that virus entry was arrested after CD4 and coreceptor engagement. By measuring the activities of various "],"journal":["Journal of virology"],"pubmed_title":["Redox-triggered infection by disulfide-shackled human immunodeficiency virus type 1 pseudovirions."],"pmcid":["PMC154040"],"funding_grant_id":["R37 AI033292","AI49566","R01 AI033292","AI33292"],"pubmed_authors":["Schulke N","Cayanan CS","Binley JM","Wiley C","Olson WC","Burton DR"],"additional_accession":[]},"is_claimable":false,"name":"Redox-triggered infection by disulfide-shackled human immunodeficiency virus type 1 pseudovirions.","description":"We previously described a human immunodeficiency virus type 1 (HIV-1) envelope mutant that introduces a disulfide bridge between the gp120 surface proteins and gp41 transmembrane proteins (J. M. Binley, R. W. Sanders, B. Clas, N. Schuelke, A. Master, Y. Guo, F. Kajumo, D. J. Anselma, P. J. Maddon, W. C. Olson, and J. P. Moore, J. Virol. 74:627-643, 2000). Here we produced pseudovirions bearing the mutant envelope and a reporter gene to examine the mutant's infectious properties. These pseudovirions attach to cells expressing CD4 and coreceptor but infect only when triggered with reducing agent, implying that gp120-gp41 dissociation is necessary for infection. Further studies suggested that virus entry was arrested after CD4 and coreceptor engagement. By measuring the activities of various ","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 May","modification":"2025-04-05T15:30:29.718Z","creation":"2019-06-05T18:49:35Z"},"accession":"S-EPMC154040","cross_references":{"pubmed":["12719560"],"doi":["10.1128/jvi.77.10.5678-5684.2003"]}}